Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11884 | 5' | -58.8 | NC_003266.2 | + | 15752 | 0.66 | 0.437242 |
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Target: 5'- gCCGCCGGc--UCUugGCGCGGGCGu--- -3' miRNA: 3'- -GGCGGCCccaAGA--UGUGCCCGCucau -5' |
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| 11884 | 5' | -58.8 | NC_003266.2 | + | 16847 | 0.66 | 0.418095 |
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Target: 5'- gCGCCGGGGUUUU-CG-GGGCGc--- -3' miRNA: 3'- gGCGGCCCCAAGAuGUgCCCGCucau -5' |
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| 11884 | 5' | -58.8 | NC_003266.2 | + | 23787 | 0.68 | 0.338609 |
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Target: 5'- gCGCuUGGGGggC-ACGCGcGGCGAGa- -3' miRNA: 3'- gGCG-GCCCCaaGaUGUGC-CCGCUCau -5' |
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| 11884 | 5' | -58.8 | NC_003266.2 | + | 16515 | 0.68 | 0.330484 |
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Target: 5'- gCGUCGGGGcUUgaaGCGCgaGGGCGAGg- -3' miRNA: 3'- gGCGGCCCCaAGa--UGUG--CCCGCUCau -5' |
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| 11884 | 5' | -58.8 | NC_003266.2 | + | 33789 | 0.68 | 0.314668 |
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Target: 5'- aC-CCGGGGUcccugcgCUGCaugGCGGGCGGGa- -3' miRNA: 3'- gGcGGCCCCAa------GAUG---UGCCCGCUCau -5' |
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| 11884 | 5' | -58.8 | NC_003266.2 | + | 13126 | 1.08 | 0.00035 |
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Target: 5'- gCCGCCGGGGUUCUACACGGGCGAGUAc -3' miRNA: 3'- -GGCGGCCCCAAGAUGUGCCCGCUCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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