Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11889 | 3' | -55.2 | NC_003266.2 | + | 11818 | 0.66 | 0.591227 |
Target: 5'- gGGCGCG-GACCugcACUGGCagcccagccGCCGGGu -3' miRNA: 3'- -UCGCGCaCUGGcaaUGACUG---------CGGUCU- -5' |
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11889 | 3' | -55.2 | NC_003266.2 | + | 12932 | 0.66 | 0.568732 |
Target: 5'- aAGUGUGgGACUGUUcCUGAUGCaGGAg -3' miRNA: 3'- -UCGCGCaCUGGCAAuGACUGCGgUCU- -5' |
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11889 | 3' | -55.2 | NC_003266.2 | + | 35826 | 0.68 | 0.492143 |
Target: 5'- uAGCGUGggaaACCGgaaaUGACGCCAGu -3' miRNA: 3'- -UCGCGCac--UGGCaaugACUGCGGUCu -5' |
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11889 | 3' | -55.2 | NC_003266.2 | + | 15904 | 0.71 | 0.298325 |
Target: 5'- cGCGCGUcuccgaguccuGCCGgcGCUGGCGCCuGAa -3' miRNA: 3'- uCGCGCAc----------UGGCaaUGACUGCGGuCU- -5' |
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11889 | 3' | -55.2 | NC_003266.2 | + | 15306 | 1.09 | 0.000631 |
Target: 5'- cAGCGCGUGACCGUUACUGACGCCAGAc -3' miRNA: 3'- -UCGCGCACUGGCAAUGACUGCGGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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