Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11890 | 3' | -55 | NC_003266.2 | + | 4449 | 0.66 | 0.564315 |
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Target: 5'- --aUGGGCCCGUGGgCg-GCGGCuuGGg -3' miRNA: 3'- agaAUUCGGGCACUgGgaCGUCGu-CC- -5' |
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| 11890 | 3' | -55 | NC_003266.2 | + | 23131 | 0.67 | 0.553106 |
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Target: 5'- uUCUU-GGUCCGaGGCCaCgcgGCGGUAGGu -3' miRNA: 3'- -AGAAuUCGGGCaCUGG-Ga--CGUCGUCC- -5' |
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| 11890 | 3' | -55 | NC_003266.2 | + | 17014 | 0.72 | 0.294088 |
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Target: 5'- -----cGCCUcUGACCCUGCcGCGGGc -3' miRNA: 3'- agaauuCGGGcACUGGGACGuCGUCC- -5' |
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| 11890 | 3' | -55 | NC_003266.2 | + | 7081 | 0.73 | 0.237885 |
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Target: 5'- --aUAGGCCUGggcGGCCUUGC-GCAGGg -3' miRNA: 3'- agaAUUCGGGCa--CUGGGACGuCGUCC- -5' |
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| 11890 | 3' | -55 | NC_003266.2 | + | 13183 | 0.74 | 0.201866 |
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Target: 5'- -----cGCCCGUGuagaACCCcgGCGGCGGGa -3' miRNA: 3'- agaauuCGGGCAC----UGGGa-CGUCGUCC- -5' |
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| 11890 | 3' | -55 | NC_003266.2 | + | 16469 | 1.11 | 0.000439 |
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Target: 5'- gUCUUAAGCCCGUGACCCUGCAGCAGGu -3' miRNA: 3'- -AGAAUUCGGGCACUGGGACGUCGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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