Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11890 | 5' | -63.8 | NC_003266.2 | + | 16555 | 0.67 | 0.180206 |
Target: 5'- cGaCGCGGCGCCGCGcUCGGcaGCa---- -3' miRNA: 3'- uC-GCGCCGCGGCGC-AGCCc-CGaacuu -5' |
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11890 | 5' | -63.8 | NC_003266.2 | + | 8465 | 0.67 | 0.175452 |
Target: 5'- gGGCacguCGGCGCCGCG-CGcGGGCa---- -3' miRNA: 3'- -UCGc---GCCGCGGCGCaGC-CCCGaacuu -5' |
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11890 | 5' | -63.8 | NC_003266.2 | + | 10871 | 0.68 | 0.149215 |
Target: 5'- cGcCGCGGcCGCCGUGagCGGGGCcgGGc -3' miRNA: 3'- uC-GCGCC-GCGGCGCa-GCCCCGaaCUu -5' |
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11890 | 5' | -63.8 | NC_003266.2 | + | 16503 | 1.05 | 0.000135 |
Target: 5'- gAGCGCGGCGCCGCGUCGGGGCUUGAAg -3' miRNA: 3'- -UCGCGCCGCGGCGCAGCCCCGAACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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