Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11892 | 5' | -59.2 | NC_003266.2 | + | 12646 | 0.66 | 0.375292 |
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Target: 5'- ---cGCGCCUgcuGCugCUGCUGGUGGc -3' miRNA: 3'- cccuCGCGGGu--UGugGACGGUCACCu -5' |
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| 11892 | 5' | -59.2 | NC_003266.2 | + | 13379 | 0.67 | 0.332757 |
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Target: 5'- aGGAucacGCGCCC-GCGCUUGCUGGgcgaGGAg -3' miRNA: 3'- cCCU----CGCGGGuUGUGGACGGUCa---CCU- -5' |
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| 11892 | 5' | -59.2 | NC_003266.2 | + | 21332 | 0.67 | 0.316762 |
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Target: 5'- aGGGAcugcuGCGCCgCAGCGCCcugGCCAccaaGGAc -3' miRNA: 3'- -CCCU-----CGCGG-GUUGUGGa--CGGUca--CCU- -5' |
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| 11892 | 5' | -59.2 | NC_003266.2 | + | 5101 | 0.67 | 0.308984 |
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Target: 5'- -uGGGCGUCCAGCGCg-GCCAG-GGu -3' miRNA: 3'- ccCUCGCGGGUUGUGgaCGGUCaCCu -5' |
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| 11892 | 5' | -59.2 | NC_003266.2 | + | 10118 | 0.68 | 0.299092 |
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Target: 5'- cGGGGGCGCCgGGCGCUagGUCcucgagcauggugcGGUGGu -3' miRNA: 3'- -CCCUCGCGGgUUGUGGa-CGG--------------UCACCu -5' |
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| 11892 | 5' | -59.2 | NC_003266.2 | + | 34800 | 0.69 | 0.258616 |
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Target: 5'- aGGGuGUGCUUAAgAa-UGCCGGUGGAg -3' miRNA: 3'- -CCCuCGCGGGUUgUggACGGUCACCU- -5' |
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| 11892 | 5' | -59.2 | NC_003266.2 | + | 13276 | 0.7 | 0.193244 |
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Target: 5'- aGGGGCGCUCGuuaGCACCcgGUCGGggGGAg -3' miRNA: 3'- cCCUCGCGGGU---UGUGGa-CGGUCa-CCU- -5' |
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| 11892 | 5' | -59.2 | NC_003266.2 | + | 21515 | 0.77 | 0.067917 |
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Target: 5'- cGGGGGUGCCCAACGgCaUGCUccagucgccccaGGUGGAa -3' miRNA: 3'- -CCCUCGCGGGUUGUgG-ACGG------------UCACCU- -5' |
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| 11892 | 5' | -59.2 | NC_003266.2 | + | 18609 | 1.11 | 0.000164 |
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Target: 5'- aGGGAGCGCCCAACACCUGCCAGUGGAa -3' miRNA: 3'- -CCCUCGCGGGUUGUGGACGGUCACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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