Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11893 | 5' | -65 | NC_003266.2 | + | 25770 | 0.66 | 0.194922 |
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Target: 5'- cGGuCUGGGUGGUGGgGUUCgggaaUCGCccGGUc -3' miRNA: 3'- -CC-GGCCCACCACCgCGGG-----AGCGa-CCA- -5' |
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| 11893 | 5' | -65 | NC_003266.2 | + | 17087 | 0.66 | 0.189925 |
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Target: 5'- uGCuCGGGUGGUaGcGCGCCCgcgGCagGGUc -3' miRNA: 3'- cCG-GCCCACCA-C-CGCGGGag-CGa-CCA- -5' |
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| 11893 | 5' | -65 | NC_003266.2 | + | 26689 | 0.66 | 0.186981 |
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Target: 5'- aGCgGGGUGGUGacgacacaggguggcGcCGCCCgccaGCUGGa -3' miRNA: 3'- cCGgCCCACCAC---------------C-GCGGGag--CGACCa -5' |
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| 11893 | 5' | -65 | NC_003266.2 | + | 13010 | 0.66 | 0.180267 |
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Target: 5'- cGCUGGG-GGUGGCcCCCUC-CUGc- -3' miRNA: 3'- cCGGCCCaCCACCGcGGGAGcGACca -5' |
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| 11893 | 5' | -65 | NC_003266.2 | + | 20883 | 0.7 | 0.091876 |
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Target: 5'- uGGCCGaGGUcGGUGagcGCGCCCauggacaugaagUUGCUGGa -3' miRNA: 3'- -CCGGC-CCA-CCAC---CGCGGG------------AGCGACCa -5' |
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| 11893 | 5' | -65 | NC_003266.2 | + | 19695 | 1.09 | 0.000069 |
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Target: 5'- cGGCCGGGUGGUGGCGCCCUCGCUGGUg -3' miRNA: 3'- -CCGGCCCACCACCGCGGGAGCGACCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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