Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11895 | 5' | -53.9 | NC_003266.2 | + | 6320 | 0.66 | 0.588738 |
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Target: 5'- gGUCCaGCAGAGgcggccgCCCUUGcGCGAgcaGAa -3' miRNA: 3'- -UAGGaCGUCUCaa-----GGGAAC-CGUUg--CU- -5' |
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| 11895 | 5' | -53.9 | NC_003266.2 | + | 25200 | 0.66 | 0.588738 |
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Target: 5'- -cCCggGguGAGUUucagacccCCCUUGGCGGCa- -3' miRNA: 3'- uaGGa-CguCUCAA--------GGGAACCGUUGcu -5' |
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| 11895 | 5' | -53.9 | NC_003266.2 | + | 23032 | 0.67 | 0.547394 |
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Target: 5'- cGUUUUGCGGGGUUUCUUUuugggcggcugcugcGGCGGCGGu -3' miRNA: 3'- -UAGGACGUCUCAAGGGAA---------------CCGUUGCU- -5' |
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| 11895 | 5' | -53.9 | NC_003266.2 | + | 9499 | 0.68 | 0.520341 |
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Target: 5'- gGUCCUugcGCGGAG-UCCCUacggGGCAAuUGAu -3' miRNA: 3'- -UAGGA---CGUCUCaAGGGAa---CCGUU-GCU- -5' |
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| 11895 | 5' | -53.9 | NC_003266.2 | + | 33956 | 0.71 | 0.339003 |
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Target: 5'- uGUCCUGCaAGAGUUCCCUgugcccugggauaUGGUccUGAc -3' miRNA: 3'- -UAGGACG-UCUCAAGGGA-------------ACCGuuGCU- -5' |
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| 11895 | 5' | -53.9 | NC_003266.2 | + | 19953 | 0.97 | 0.005225 |
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Target: 5'- gAUCCUGC-GAGUUCCCUUGGCAACGAc -3' miRNA: 3'- -UAGGACGuCUCAAGGGAACCGUUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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