Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11897 | 3' | -47.7 | NC_003266.2 | + | 15887 | 0.66 | 0.95603 |
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Target: 5'- uGCCGGcgcUGGCGCCugaaGCCGCGcg---UCUg -3' miRNA: 3'- -CGGCC---AUUGCGG----UGGUGUauuuaAGA- -5' |
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| 11897 | 3' | -47.7 | NC_003266.2 | + | 13559 | 0.66 | 0.95603 |
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Target: 5'- aGCCGGggcagUGCCGCC-CGUAAAc--- -3' miRNA: 3'- -CGGCCauu--GCGGUGGuGUAUUUaaga -5' |
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| 11897 | 3' | -47.7 | NC_003266.2 | + | 20147 | 0.67 | 0.935599 |
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Target: 5'- cCCGGccAACGCCACCaACGUGc----- -3' miRNA: 3'- cGGCCa-UUGCGGUGG-UGUAUuuaaga -5' |
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| 11897 | 3' | -47.7 | NC_003266.2 | + | 18427 | 0.67 | 0.916868 |
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Target: 5'- cGUgGGUAgGCGCCACCGug-GGGUUCc -3' miRNA: 3'- -CGgCCAU-UGCGGUGGUguaUUUAAGa -5' |
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| 11897 | 3' | -47.7 | NC_003266.2 | + | 3604 | 0.67 | 0.916193 |
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Target: 5'- cGUCGGUGGacgcagcUGCCGCCGCAgcugcugcUUCUg -3' miRNA: 3'- -CGGCCAUU-------GCGGUGGUGUauuu----AAGA- -5' |
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| 11897 | 3' | -47.7 | NC_003266.2 | + | 26013 | 0.67 | 0.909971 |
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Target: 5'- uGCCGc---CGCCGCCGCuGUGGAUUUUc -3' miRNA: 3'- -CGGCcauuGCGGUGGUG-UAUUUAAGA- -5' |
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| 11897 | 3' | -47.7 | NC_003266.2 | + | 22363 | 0.69 | 0.851081 |
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Target: 5'- cGCCGGguGCGCUACCAaccaguuUUCUa -3' miRNA: 3'- -CGGCCauUGCGGUGGUguauuu-AAGA- -5' |
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| 11897 | 3' | -47.7 | NC_003266.2 | + | 16846 | 0.69 | 0.843606 |
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Target: 5'- cGCCGGgguuuucggGGCGCCGCCgGCAUccaaGGAUccgUCUg -3' miRNA: 3'- -CGGCCa--------UUGCGGUGG-UGUA----UUUA---AGA- -5' |
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| 11897 | 3' | -47.7 | NC_003266.2 | + | 15768 | 0.72 | 0.667741 |
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Target: 5'- cGCCGGgcgauGCGCCGCCGCcgGc--UCUu -3' miRNA: 3'- -CGGCCau---UGCGGUGGUGuaUuuaAGA- -5' |
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| 11897 | 3' | -47.7 | NC_003266.2 | + | 21016 | 1.11 | 0.002838 |
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Target: 5'- aGCCGGUAACGCCACCACAUAAAUUCUu -3' miRNA: 3'- -CGGCCAUUGCGGUGGUGUAUUUAAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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