Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
119 | 5' | -61.6 | AC_000006.1 | + | 4779 | 0.66 | 0.290327 |
Target: 5'- gUCCCGGAgGAGgggggccacCUCGuuGAgcaugucucuaacuuGGAGGUn -3' miRNA: 3'- aAGGGCCUgCUC---------GAGCggCU---------------CCUCCG- -5' |
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119 | 5' | -61.6 | AC_000006.1 | + | 6437 | 0.66 | 0.287447 |
Target: 5'- cUCgCGGGCGGccauuGCUCGCUcguagggguuGAGG-GGCg -3' miRNA: 3'- aAGgGCCUGCU-----CGAGCGG----------CUCCuCCG- -5' |
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119 | 5' | -61.6 | AC_000006.1 | + | 17586 | 0.67 | 0.259873 |
Target: 5'- -cUUCGGugGAGCgggCGUgGGugguGGAGGCa -3' miRNA: 3'- aaGGGCCugCUCGa--GCGgCU----CCUCCG- -5' |
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119 | 5' | -61.6 | AC_000006.1 | + | 26242 | 0.67 | 0.240637 |
Target: 5'- cUCCCGGGCGaAGCagCGCaGGcGGcGGCa -3' miRNA: 3'- aAGGGCCUGC-UCGa-GCGgCU-CCuCCG- -5' |
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119 | 5' | -61.6 | AC_000006.1 | + | 9018 | 0.68 | 0.216854 |
Target: 5'- --gCCuGAUGAGCUCGgCGAuGGuGGCg -3' miRNA: 3'- aagGGcCUGCUCGAGCgGCU-CCuCCG- -5' |
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119 | 5' | -61.6 | AC_000006.1 | + | 8092 | 0.69 | 0.186961 |
Target: 5'- cUCCCGcGCGGGCUCGUcagccucuccguccuCGAGGAGc- -3' miRNA: 3'- aAGGGCcUGCUCGAGCG---------------GCUCCUCcg -5' |
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119 | 5' | -61.6 | AC_000006.1 | + | 26141 | 0.69 | 0.180077 |
Target: 5'- -aCUCGGcUGAGCUCGCuCGGuuGAGGCa -3' miRNA: 3'- aaGGGCCuGCUCGAGCG-GCUc-CUCCG- -5' |
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119 | 5' | -61.6 | AC_000006.1 | + | 14191 | 0.69 | 0.170634 |
Target: 5'- -aCCCGGACGugguGCUgcUGCCGGGcugcGGGGUg -3' miRNA: 3'- aaGGGCCUGCu---CGA--GCGGCUC----CUCCG- -5' |
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119 | 5' | -61.6 | AC_000006.1 | + | 10704 | 0.7 | 0.140985 |
Target: 5'- -gCCgCGGucGCGGuGgUCGCCGGGGGGGUg -3' miRNA: 3'- aaGG-GCC--UGCU-CgAGCGGCUCCUCCG- -5' |
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119 | 5' | -61.6 | AC_000006.1 | + | 12669 | 0.7 | 0.137155 |
Target: 5'- aUCCCGGcgcaguACGcGCUgucgGCCGAGGAGGa -3' miRNA: 3'- aAGGGCC------UGCuCGAg---CGGCUCCUCCg -5' |
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119 | 5' | -61.6 | AC_000006.1 | + | 17406 | 0.75 | 0.062017 |
Target: 5'- aUCCUgcagguGGACGAGC-CGCCuucguacGAGGAGGCc -3' miRNA: 3'- aAGGG------CCUGCUCGaGCGG-------CUCCUCCG- -5' |
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119 | 5' | -61.6 | AC_000006.1 | + | 26331 | 0.8 | 0.026769 |
Target: 5'- cUUCCUGGuCGAGCggGaCCGGGGAGGCa -3' miRNA: 3'- -AAGGGCCuGCUCGagC-GGCUCCUCCG- -5' |
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119 | 5' | -61.6 | AC_000006.1 | + | 4829 | 1.09 | 0.000129 |
Target: 5'- uUUCCCGGACGAGCUCGCCGAGGAGGCg -3' miRNA: 3'- -AAGGGCCUGCUCGAGCGGCUCCUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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