miRNA: 3'- gGGGGUccuACGGGgCUCCUUCGUCG- -5'
miRNA: 3'- ggGGGUC-CUaCGGGGCUC-CUUCGUCG- -5'
miRNA: 3'- -GGGGGUCCUACGGGGCUCCUUCGUCG- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11901 | 3' | -60 | NC_003266.2 | + | 17826 | 0.72 | 0.153493 |
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Target: 5'- gCCCCGcu-UGUCCaGGGGAGGCAGCu -3' miRNA: 3'- gGGGGUccuACGGGgCUCCUUCGUCG- -5' |
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| 11901 | 3' | -60 | NC_003266.2 | + | 27103 | 0.74 | 0.098252 |
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Target: 5'- -aCUCGGcGAgGUCCCGAGcGAAGCAGCg -3' miRNA: 3'- ggGGGUC-CUaCGGGGCUC-CUUCGUCG- -5' |
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| 11901 | 3' | -60 | NC_003266.2 | + | 25405 | 1.13 | 0.000102 |
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Target: 5'- uCCCCCAGGAUGCCCCGAGGAAGCAGCa -3' miRNA: 3'- -GGGGGUCCUACGGGGCUCCUUCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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