miRNA: 3'- -CUCGA-CGGcgGGCACCUCCUaaa-CCU- -5'
miRNA: 3'- -CUCgACGGCGggCACCUCCUaaaCCU- -5'
miRNA: 3'- cuCGaCGGCGGgCACCUCCuaaACCu -5'
miRNA: 3'- -CUCGACGGCG--GgCACCUCCUAaaCCU- -5'
miRNA: 3'- -CUCGACGGCGGGCACCUCCUAAACCU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11901 | 5' | -57.8 | NC_003266.2 | + | 18036 | 0.66 | 0.432453 |
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Target: 5'- gGGGCUaGCCa-CUGUGGAGGGgcggGGAg -3' miRNA: 3'- -CUCGA-CGGcgGGCACCUCCUaaa-CCU- -5' |
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| 11901 | 5' | -57.8 | NC_003266.2 | + | 17743 | 0.67 | 0.366928 |
|
Target: 5'- gGAGaUGCCGCagGUGGAGGA---GGAg -3' miRNA: 3'- -CUCgACGGCGggCACCUCCUaaaCCU- -5' |
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| 11901 | 5' | -57.8 | NC_003266.2 | + | 17233 | 0.67 | 0.349503 |
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Target: 5'- -cGCcGCCGCCgGUGGGGG---UGGc -3' miRNA: 3'- cuCGaCGGCGGgCACCUCCuaaACCu -5' |
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| 11901 | 5' | -57.8 | NC_003266.2 | + | 10844 | 0.75 | 0.115353 |
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Target: 5'- gGGGCUGCUGUugCgGUGGAGGGUggGGGc -3' miRNA: 3'- -CUCGACGGCG--GgCACCUCCUAaaCCU- -5' |
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| 11901 | 5' | -57.8 | NC_003266.2 | + | 25446 | 1.08 | 0.000313 |
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Target: 5'- gGAGCUGCCGCCCGUGGAGGAUUUGGAg -3' miRNA: 3'- -CUCGACGGCGGGCACCUCCUAAACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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