Results 1 - 3 of 3 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11902 | 3' | -62.1 | NC_003266.2 | + | 25163 | 0.66 | 0.252999 |
Target: 5'- -cCUCAuCGCCGGGCCCuugcaacucgaaGGugccGAUGAUCu -3' miRNA: 3'- ccGAGUcGCGGCCCGGG------------CC----CUACUAG- -5' |
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11902 | 3' | -62.1 | NC_003266.2 | + | 25302 | 0.67 | 0.240398 |
Target: 5'- aGCUCGGCcUUGGGCggCUGGGAUuGGUCc -3' miRNA: 3'- cCGAGUCGcGGCCCG--GGCCCUA-CUAG- -5' |
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11902 | 3' | -62.1 | NC_003266.2 | + | 26391 | 1.1 | 0.000101 |
Target: 5'- uGGCUCAGCGCCGGGCCCGGGAUGAUCu -3' miRNA: 3'- -CCGAGUCGCGGCCCGGGCCCUACUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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