Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11903 | 5' | -55.1 | NC_003266.2 | + | 16225 | 0.66 | 0.580561 |
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Target: 5'- gUGCAGUGGCGcggGCGGAaagugcGUCCggUGCUGAGa -3' miRNA: 3'- -ACGUUGCUGC---UGCCU------CAGG--ACGACUU- -5' |
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| 11903 | 5' | -55.1 | NC_003266.2 | + | 12210 | 0.66 | 0.535472 |
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Target: 5'- cGCGGCGACGAggccgggcUGGuGUacaacgcaCUGCUGGAg -3' miRNA: 3'- aCGUUGCUGCU--------GCCuCAg-------GACGACUU- -5' |
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| 11903 | 5' | -55.1 | NC_003266.2 | + | 23375 | 0.68 | 0.459937 |
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Target: 5'- gGCGACGAUGGCuGAGUCUccaUGCUu-- -3' miRNA: 3'- aCGUUGCUGCUGcCUCAGG---ACGAcuu -5' |
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| 11903 | 5' | -55.1 | NC_003266.2 | + | 16810 | 0.68 | 0.439395 |
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Target: 5'- cGCAaguACGGCG-CGGccAG-CCUGCUGAu -3' miRNA: 3'- aCGU---UGCUGCuGCC--UCaGGACGACUu -5' |
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| 11903 | 5' | -55.1 | NC_003266.2 | + | 8525 | 0.7 | 0.353925 |
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Target: 5'- aGCGACGacGCGACGGuugacGUCCUGgauCUGAc -3' miRNA: 3'- aCGUUGC--UGCUGCCu----CAGGAC---GACUu -5' |
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| 11903 | 5' | -55.1 | NC_003266.2 | + | 13867 | 0.7 | 0.336595 |
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Target: 5'- gGCGGCGGCGGCGGcgaugcAGcCCccGCUGGAg -3' miRNA: 3'- aCGUUGCUGCUGCC------UCaGGa-CGACUU- -5' |
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| 11903 | 5' | -55.1 | NC_003266.2 | + | 9828 | 0.72 | 0.239174 |
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Target: 5'- gGuCAGCGACGACGcgcucggcGAGgauggCCUGCUGGAu -3' miRNA: 3'- aC-GUUGCUGCUGC--------CUCa----GGACGACUU- -5' |
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| 11903 | 5' | -55.1 | NC_003266.2 | + | 16996 | 0.75 | 0.161783 |
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Target: 5'- gUGCGGCGACGACGGcggcgggugguGGUCUUGCg--- -3' miRNA: 3'- -ACGUUGCUGCUGCC-----------UCAGGACGacuu -5' |
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| 11903 | 5' | -55.1 | NC_003266.2 | + | 27455 | 1.06 | 0.000897 |
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Target: 5'- cUGCAACGACGACGGAGUCCUGCUGAAc -3' miRNA: 3'- -ACGUUGCUGCUGCCUCAGGACGACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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