Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11909 | 3' | -52.8 | NC_003278.1 | + | 6269 | 0.66 | 0.738038 |
Target: 5'- uGCCggugaagaucgCACC--GGCggCCACGCCGAg -3' miRNA: 3'- gCGGaa---------GUGGaaCUGaaGGUGUGGCU- -5' |
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11909 | 3' | -52.8 | NC_003278.1 | + | 7241 | 0.66 | 0.718046 |
Target: 5'- gGCCUggCGCCUUGAgaagaucgcggcCggCUAUGCCGAa -3' miRNA: 3'- gCGGAa-GUGGAACU------------GaaGGUGUGGCU- -5' |
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11909 | 3' | -52.8 | NC_003278.1 | + | 16491 | 0.67 | 0.69548 |
Target: 5'- uGCCUUCGCCc--GCUUCUGCagcaaaaaACCGGc -3' miRNA: 3'- gCGGAAGUGGaacUGAAGGUG--------UGGCU- -5' |
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11909 | 3' | -52.8 | NC_003278.1 | + | 2891 | 0.67 | 0.68409 |
Target: 5'- uGCCUUgGCCgggcGAC-UUgACGCCGAu -3' miRNA: 3'- gCGGAAgUGGaa--CUGaAGgUGUGGCU- -5' |
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11909 | 3' | -52.8 | NC_003278.1 | + | 13842 | 0.67 | 0.649642 |
Target: 5'- gGCCUgCGCCggGGCgcUgGCGCCGAg -3' miRNA: 3'- gCGGAaGUGGaaCUGaaGgUGUGGCU- -5' |
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11909 | 3' | -52.8 | NC_003278.1 | + | 18043 | 0.67 | 0.638108 |
Target: 5'- gGCCagaUCACCaUGGCUUCgCGCGCggCGAa -3' miRNA: 3'- gCGGa--AGUGGaACUGAAG-GUGUG--GCU- -5' |
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11909 | 3' | -52.8 | NC_003278.1 | + | 21359 | 0.68 | 0.615033 |
Target: 5'- gGCCUcgcUCGCCgcgaucagcGCUgCCGCGCCGAu -3' miRNA: 3'- gCGGA---AGUGGaac------UGAaGGUGUGGCU- -5' |
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11909 | 3' | -52.8 | NC_003278.1 | + | 5793 | 0.69 | 0.524183 |
Target: 5'- cCGCCUuggUCAUCUUcGACgggUCCugGCCu- -3' miRNA: 3'- -GCGGA---AGUGGAA-CUGa--AGGugUGGcu -5' |
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11909 | 3' | -52.8 | NC_003278.1 | + | 33174 | 0.7 | 0.513143 |
Target: 5'- gGCCUUCACCUucggUGA--UCCgAUGCCGGu -3' miRNA: 3'- gCGGAAGUGGA----ACUgaAGG-UGUGGCU- -5' |
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11909 | 3' | -52.8 | NC_003278.1 | + | 20490 | 0.7 | 0.513143 |
Target: 5'- gGCgUUCACCgUGACUUUCGCGuaggucCCGGc -3' miRNA: 3'- gCGgAAGUGGaACUGAAGGUGU------GGCU- -5' |
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11909 | 3' | -52.8 | NC_003278.1 | + | 21227 | 0.7 | 0.480627 |
Target: 5'- gCGCCUUCGCCggcguccugGGCUaCCuggGCAUCGAc -3' miRNA: 3'- -GCGGAAGUGGaa-------CUGAaGG---UGUGGCU- -5' |
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11909 | 3' | -52.8 | NC_003278.1 | + | 18484 | 0.7 | 0.480627 |
Target: 5'- gCGUCUUCGgCggUGGCUaCCAgGCCGAu -3' miRNA: 3'- -GCGGAAGUgGa-ACUGAaGGUgUGGCU- -5' |
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11909 | 3' | -52.8 | NC_003278.1 | + | 26284 | 0.7 | 0.470012 |
Target: 5'- aGCCUUCagcGCCUUGugUa--GCGCCGGg -3' miRNA: 3'- gCGGAAG---UGGAACugAaggUGUGGCU- -5' |
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11909 | 3' | -52.8 | NC_003278.1 | + | 12612 | 0.71 | 0.43891 |
Target: 5'- gGCUUUCACggUGGCcaCCGCAUCGAa -3' miRNA: 3'- gCGGAAGUGgaACUGaaGGUGUGGCU- -5' |
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11909 | 3' | -52.8 | NC_003278.1 | + | 34846 | 1.09 | 0.001055 |
Target: 5'- uCGCCUUCACCUUGACUUCCACACCGAc -3' miRNA: 3'- -GCGGAAGUGGAACUGAAGGUGUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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