Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11910 | 3' | -58.9 | NC_003278.1 | + | 1164 | 0.66 | 0.361217 |
Target: 5'- --uAGCUUGGCCUgcugcugcaGUGcgcgcagcGCCGGGGCc -3' miRNA: 3'- guuUCGGACCGGAa--------CAC--------CGGUCCCGa -5' |
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11910 | 3' | -58.9 | NC_003278.1 | + | 16544 | 0.66 | 0.357703 |
Target: 5'- gGAGGUC-GGCCggguccaugccgGUGGCCucGGGCUc -3' miRNA: 3'- gUUUCGGaCCGGaa----------CACCGGu-CCCGA- -5' |
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11910 | 3' | -58.9 | NC_003278.1 | + | 29897 | 0.66 | 0.330459 |
Target: 5'- gAGGGCCUGGCgCUUGaGGaucgcgucgcgcagCAGGGCc -3' miRNA: 3'- gUUUCGGACCG-GAACaCCg-------------GUCCCGa -5' |
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11910 | 3' | -58.9 | NC_003278.1 | + | 31740 | 0.66 | 0.327163 |
Target: 5'- uCGAGcGCCUGGCCg-GUGuCCcGGGCa -3' miRNA: 3'- -GUUU-CGGACCGGaaCACcGGuCCCGa -5' |
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11910 | 3' | -58.9 | NC_003278.1 | + | 12564 | 0.67 | 0.303222 |
Target: 5'- cCAGAaCCUGGCC----GGCCAGGuGCUc -3' miRNA: 3'- -GUUUcGGACCGGaacaCCGGUCC-CGA- -5' |
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11910 | 3' | -58.9 | NC_003278.1 | + | 14056 | 0.67 | 0.288023 |
Target: 5'- --cGGCCUGGCCggcgccgaGGCCcuGGGCc -3' miRNA: 3'- guuUCGGACCGGaaca----CCGGu-CCCGa -5' |
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11910 | 3' | -58.9 | NC_003278.1 | + | 9853 | 0.67 | 0.280651 |
Target: 5'- --cGGCCUGGCCUg--GGCCgAGuGCg -3' miRNA: 3'- guuUCGGACCGGAacaCCGG-UCcCGa -5' |
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11910 | 3' | -58.9 | NC_003278.1 | + | 21519 | 0.68 | 0.27343 |
Target: 5'- cCAAGGCCUGGUCagcgcgGGCCGucaGGCUg -3' miRNA: 3'- -GUUUCGGACCGGaaca--CCGGUc--CCGA- -5' |
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11910 | 3' | -58.9 | NC_003278.1 | + | 25201 | 0.68 | 0.266359 |
Target: 5'- --cGGCCcaucgaccucgaUGGCCUcgGUGaGCUGGGGCUc -3' miRNA: 3'- guuUCGG------------ACCGGAa-CAC-CGGUCCCGA- -5' |
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11910 | 3' | -58.9 | NC_003278.1 | + | 21456 | 0.68 | 0.252661 |
Target: 5'- --cGGCCUGGCCcag-GGCCucGGCg -3' miRNA: 3'- guuUCGGACCGGaacaCCGGucCCGa -5' |
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11910 | 3' | -58.9 | NC_003278.1 | + | 1304 | 0.68 | 0.239547 |
Target: 5'- aCAAAGcCCUGGCCacaaGGCCAGG-CUu -3' miRNA: 3'- -GUUUC-GGACCGGaacaCCGGUCCcGA- -5' |
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11910 | 3' | -58.9 | NC_003278.1 | + | 25309 | 0.69 | 0.212698 |
Target: 5'- cCAAAGCCUcauaGGCCUUGaagcagguuucguUGGCgcugcugcggauacCAGGGCg -3' miRNA: 3'- -GUUUCGGA----CCGGAAC-------------ACCG--------------GUCCCGa -5' |
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11910 | 3' | -58.9 | NC_003278.1 | + | 22140 | 0.71 | 0.149764 |
Target: 5'- --cGGCCUGGCCca-UGGUCgAGGGCa -3' miRNA: 3'- guuUCGGACCGGaacACCGG-UCCCGa -5' |
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11910 | 3' | -58.9 | NC_003278.1 | + | 21019 | 0.72 | 0.133627 |
Target: 5'- uGGGGCCUGGCCgccaacccgGUGGUCcuGGCa -3' miRNA: 3'- gUUUCGGACCGGaa-------CACCGGucCCGa -5' |
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11910 | 3' | -58.9 | NC_003278.1 | + | 34212 | 1.07 | 0.00026 |
Target: 5'- aCAAAGCCUGGCCUUGUGGCCAGGGCUu -3' miRNA: 3'- -GUUUCGGACCGGAACACCGGUCCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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