Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11912 | 3' | -54.6 | NC_003278.1 | + | 14681 | 0.66 | 0.604547 |
Target: 5'- cGGGCCGAGaaug-CCGgCGAgGGUGAUg -3' miRNA: 3'- -UCCGGCUCaaguaGGC-GUU-CCACUGg -5' |
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11912 | 3' | -54.6 | NC_003278.1 | + | 20579 | 0.66 | 0.615919 |
Target: 5'- gAGGgCGAGg----CCGCGAGGgucGCCg -3' miRNA: 3'- -UCCgGCUCaaguaGGCGUUCCac-UGG- -5' |
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11912 | 3' | -54.6 | NC_003278.1 | + | 5006 | 0.66 | 0.627306 |
Target: 5'- -cGCCGAGcaCAgCCGCGaccuGGGUGAgCa -3' miRNA: 3'- ucCGGCUCaaGUaGGCGU----UCCACUgG- -5' |
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11912 | 3' | -54.6 | NC_003278.1 | + | 25471 | 0.66 | 0.627306 |
Target: 5'- cGGCCGAG-UCAgccaCCGCAAcacacgaGACCa -3' miRNA: 3'- uCCGGCUCaAGUa---GGCGUUcca----CUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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