Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11913 | 3' | -57.7 | NC_003278.1 | + | 25946 | 0.66 | 0.433405 |
Target: 5'- gGUCAggucggccugggccUGGCGCUC-GCGGgaAAGGGCGGc -3' miRNA: 3'- -CAGU--------------ACUGCGGGaCGUCg-UUCCCGCU- -5' |
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11913 | 3' | -57.7 | NC_003278.1 | + | 3666 | 0.66 | 0.4265 |
Target: 5'- ----cGGCGCCCgccgGCGGCGc-GGCGGa -3' miRNA: 3'- caguaCUGCGGGa---CGUCGUucCCGCU- -5' |
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11913 | 3' | -57.7 | NC_003278.1 | + | 13834 | 0.66 | 0.416749 |
Target: 5'- aUgGUGAUGgCCUGC-GCcGGGGCGc -3' miRNA: 3'- cAgUACUGCgGGACGuCGuUCCCGCu -5' |
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11913 | 3' | -57.7 | NC_003278.1 | + | 34051 | 0.66 | 0.41385 |
Target: 5'- ----aGACGCgCCUGCAGCAGGuuaaugagaugaucGGgGAg -3' miRNA: 3'- caguaCUGCG-GGACGUCGUUC--------------CCgCU- -5' |
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11913 | 3' | -57.7 | NC_003278.1 | + | 12034 | 0.66 | 0.404278 |
Target: 5'- ----cGACGCCCUGCAucgcaccaccuaccGCGAcGGcGCGGu -3' miRNA: 3'- caguaCUGCGGGACGU--------------CGUU-CC-CGCU- -5' |
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11913 | 3' | -57.7 | NC_003278.1 | + | 29810 | 0.66 | 0.397662 |
Target: 5'- --aAUGuucACGUCCUGCAGCAGcGGGUu- -3' miRNA: 3'- cagUAC---UGCGGGACGUCGUU-CCCGcu -5' |
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11913 | 3' | -57.7 | NC_003278.1 | + | 19531 | 0.67 | 0.379146 |
Target: 5'- -aCAgccACGCCCUGCAGCAAcuGCGc -3' miRNA: 3'- caGUac-UGCGGGACGUCGUUccCGCu -5' |
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11913 | 3' | -57.7 | NC_003278.1 | + | 25337 | 0.67 | 0.379146 |
Target: 5'- uUCGuUGGCGCUgCUGCGGauaccAGGGCGGu -3' miRNA: 3'- cAGU-ACUGCGG-GACGUCgu---UCCCGCU- -5' |
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11913 | 3' | -57.7 | NC_003278.1 | + | 6476 | 0.67 | 0.361217 |
Target: 5'- ----cGGCGUgCUGCaggccGGCAAGGGCGc -3' miRNA: 3'- caguaCUGCGgGACG-----UCGUUCCCGCu -5' |
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11913 | 3' | -57.7 | NC_003278.1 | + | 13160 | 0.68 | 0.31903 |
Target: 5'- gGUCAgguaGCCCgucacGCgGGCGAGGGCGGc -3' miRNA: 3'- -CAGUacugCGGGa----CG-UCGUUCCCGCU- -5' |
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11913 | 3' | -57.7 | NC_003278.1 | + | 32594 | 0.71 | 0.203595 |
Target: 5'- gGUgGUGGCGCCUccgGCGGUAccGGGCGGc -3' miRNA: 3'- -CAgUACUGCGGGa--CGUCGUu-CCCGCU- -5' |
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11913 | 3' | -57.7 | NC_003278.1 | + | 26742 | 0.71 | 0.187435 |
Target: 5'- ----cGGCGUUCUGCAGCAGGuGCGAg -3' miRNA: 3'- caguaCUGCGGGACGUCGUUCcCGCU- -5' |
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11913 | 3' | -57.7 | NC_003278.1 | + | 29048 | 0.72 | 0.16301 |
Target: 5'- ----cGACaagGCCCUGCAGCAAGccGGCGGc -3' miRNA: 3'- caguaCUG---CGGGACGUCGUUC--CCGCU- -5' |
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11913 | 3' | -57.7 | NC_003278.1 | + | 9780 | 0.78 | 0.06055 |
Target: 5'- -cCGUGACGCCCUGCAGCcuGGagucaGCGGc -3' miRNA: 3'- caGUACUGCGGGACGUCGuuCC-----CGCU- -5' |
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11913 | 3' | -57.7 | NC_003278.1 | + | 32259 | 1.08 | 0.000303 |
Target: 5'- aGUCAUGACGCCCUGCAGCAAGGGCGAc -3' miRNA: 3'- -CAGUACUGCGGGACGUCGUUCCCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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