Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11914 | 5' | -54.6 | NC_003278.1 | + | 29891 | 0.66 | 0.646558 |
Target: 5'- cGcGUugGGcguGACCGGCCaGAagACCgCCGg -3' miRNA: 3'- aC-CAugCCu--UUGGCUGGaCU--UGG-GGC- -5' |
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11914 | 5' | -54.6 | NC_003278.1 | + | 14049 | 0.66 | 0.623939 |
Target: 5'- ---aGCGG--GCCGGCCUGGccggcgccgagGCCCUGg -3' miRNA: 3'- accaUGCCuuUGGCUGGACU-----------UGGGGC- -5' |
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11914 | 5' | -54.6 | NC_003278.1 | + | 19173 | 0.67 | 0.612635 |
Target: 5'- gGGUACGGAGuuccGCCGAuggcgaaugaCCUGcAGCUgCGc -3' miRNA: 3'- aCCAUGCCUU----UGGCU----------GGAC-UUGGgGC- -5' |
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11914 | 5' | -54.6 | NC_003278.1 | + | 16021 | 0.67 | 0.612635 |
Target: 5'- aGGUGCugcugcuugaGGAAACCGGCUUcacGGAUggCCCGg -3' miRNA: 3'- aCCAUG----------CCUUUGGCUGGA---CUUG--GGGC- -5' |
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11914 | 5' | -54.6 | NC_003278.1 | + | 30701 | 0.67 | 0.60135 |
Target: 5'- gGGUGuCGGGAuuggucuugcgGCUGGCgaGggUCCCGu -3' miRNA: 3'- aCCAU-GCCUU-----------UGGCUGgaCuuGGGGC- -5' |
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11914 | 5' | -54.6 | NC_003278.1 | + | 26608 | 0.67 | 0.578872 |
Target: 5'- cGG-ACGcGAAugCGccgccgcccGCCUGGGCCUCGa -3' miRNA: 3'- aCCaUGC-CUUugGC---------UGGACUUGGGGC- -5' |
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11914 | 5' | -54.6 | NC_003278.1 | + | 14454 | 0.67 | 0.567699 |
Target: 5'- aGGUACGcGgcGCCGGCCaGcACCgCCa -3' miRNA: 3'- aCCAUGC-CuuUGGCUGGaCuUGG-GGc -5' |
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11914 | 5' | -54.6 | NC_003278.1 | + | 7805 | 0.67 | 0.553256 |
Target: 5'- cGGUccucgaggcgaaccACGGccuGGCCGACCacGGCCCCa -3' miRNA: 3'- aCCA--------------UGCCu--UUGGCUGGacUUGGGGc -5' |
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11914 | 5' | -54.6 | NC_003278.1 | + | 18742 | 0.68 | 0.523638 |
Target: 5'- cGGUguucugcgaGCGGAAACUcACC-GGACUCCGg -3' miRNA: 3'- aCCA---------UGCCUUUGGcUGGaCUUGGGGC- -5' |
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11914 | 5' | -54.6 | NC_003278.1 | + | 3811 | 0.68 | 0.502095 |
Target: 5'- ---cGCGGAGGCCGGCC-GggUCCaCGu -3' miRNA: 3'- accaUGCCUUUGGCUGGaCuuGGG-GC- -5' |
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11914 | 5' | -54.6 | NC_003278.1 | + | 14391 | 0.7 | 0.430106 |
Target: 5'- cUGGaUACgGGAGAUCGGCCUcgaGGACgCCGa -3' miRNA: 3'- -ACC-AUG-CCUUUGGCUGGA---CUUGgGGC- -5' |
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11914 | 5' | -54.6 | NC_003278.1 | + | 18136 | 0.7 | 0.410669 |
Target: 5'- cGG-ACgGGGAGCCGACCcaccUGAuUCCCGg -3' miRNA: 3'- aCCaUG-CCUUUGGCUGG----ACUuGGGGC- -5' |
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11914 | 5' | -54.6 | NC_003278.1 | + | 21436 | 0.7 | 0.401157 |
Target: 5'- cUGG-GCGGGuucaccauGGCCGGCCUGGcccaggGCCUCGg -3' miRNA: 3'- -ACCaUGCCU--------UUGGCUGGACU------UGGGGC- -5' |
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11914 | 5' | -54.6 | NC_003278.1 | + | 31625 | 1.1 | 0.00069 |
Target: 5'- gUGGUACGGAAACCGACCUGAACCCCGa -3' miRNA: 3'- -ACCAUGCCUUUGGCUGGACUUGGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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