Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11915 | 5' | -50.4 | NC_003278.1 | + | 4851 | 0.66 | 0.875998 |
Target: 5'- cAGCGCUGCCGaaGAGGgcaCCC-UCg -3' miRNA: 3'- cUUGCGACGGCagCUUUaa-GGGuAGg -5' |
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11915 | 5' | -50.4 | NC_003278.1 | + | 15431 | 0.66 | 0.867618 |
Target: 5'- uGAACGCuUGCCGUUGGug-UCgCAgggacuggCCa -3' miRNA: 3'- -CUUGCG-ACGGCAGCUuuaAGgGUa-------GG- -5' |
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11915 | 5' | -50.4 | NC_003278.1 | + | 25342 | 0.66 | 0.85896 |
Target: 5'- uGGCGCUGCUG-CGGAuaccagggcgGUaCCgGUCCa -3' miRNA: 3'- cUUGCGACGGCaGCUU----------UAaGGgUAGG- -5' |
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11915 | 5' | -50.4 | NC_003278.1 | + | 22325 | 0.66 | 0.85896 |
Target: 5'- gGGGCGCUGCCGUUG-----CCCugggcggCCu -3' miRNA: 3'- -CUUGCGACGGCAGCuuuaaGGGua-----GG- -5' |
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11915 | 5' | -50.4 | NC_003278.1 | + | 30926 | 0.67 | 0.831415 |
Target: 5'- cAGCGCUGU--UCGAGcgUgCCUGUCCg -3' miRNA: 3'- cUUGCGACGgcAGCUUuaA-GGGUAGG- -5' |
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11915 | 5' | -50.4 | NC_003278.1 | + | 24942 | 0.67 | 0.820765 |
Target: 5'- aGGCGCUG-CGUCGggGUugcgcugUgCCAUCa -3' miRNA: 3'- cUUGCGACgGCAGCuuUA-------AgGGUAGg -5' |
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11915 | 5' | -50.4 | NC_003278.1 | + | 32198 | 0.68 | 0.780931 |
Target: 5'- uGGCGCgGCaCGUCGAGAaagugccgCCCuUCCu -3' miRNA: 3'- cUUGCGaCG-GCAGCUUUaa------GGGuAGG- -5' |
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11915 | 5' | -50.4 | NC_003278.1 | + | 21457 | 0.68 | 0.759424 |
Target: 5'- aGggUGUcGCCGUCGAucgUCgCCAggCCg -3' miRNA: 3'- -CuuGCGaCGGCAGCUuuaAG-GGUa-GG- -5' |
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11915 | 5' | -50.4 | NC_003278.1 | + | 20523 | 0.7 | 0.656931 |
Target: 5'- -uGCGC-GCCGUCGAGGgcggCCaguUCCa -3' miRNA: 3'- cuUGCGaCGGCAGCUUUaa--GGgu-AGG- -5' |
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11915 | 5' | -50.4 | NC_003278.1 | + | 1965 | 0.7 | 0.645237 |
Target: 5'- -cACGCUGCCcuucUCGAAuucgugCUCAUCCu -3' miRNA: 3'- cuUGCGACGGc---AGCUUuaa---GGGUAGG- -5' |
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11915 | 5' | -50.4 | NC_003278.1 | + | 4061 | 0.75 | 0.373503 |
Target: 5'- -uACGCUGUCGgccCGG---UCCCAUCCg -3' miRNA: 3'- cuUGCGACGGCa--GCUuuaAGGGUAGG- -5' |
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11915 | 5' | -50.4 | NC_003278.1 | + | 866 | 0.75 | 0.355171 |
Target: 5'- uAAUGCUugaagaucaucGCCGUCGAGGUgugcCCCAUCUg -3' miRNA: 3'- cUUGCGA-----------CGGCAGCUUUAa---GGGUAGG- -5' |
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11915 | 5' | -50.4 | NC_003278.1 | + | 31322 | 1.13 | 0.000972 |
Target: 5'- uGAACGCUGCCGUCGAAAUUCCCAUCCg -3' miRNA: 3'- -CUUGCGACGGCAGCUUUAAGGGUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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