Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11916 | 3' | -54.9 | NC_003278.1 | + | 33620 | 0.66 | 0.604885 |
Target: 5'- aCGGagUGCCGGagUGGUUCUg---CGCCc -3' miRNA: 3'- -GCCa-ACGGCCg-ACCAGGAacaaGUGG- -5' |
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11916 | 3' | -54.9 | NC_003278.1 | + | 16090 | 0.66 | 0.603754 |
Target: 5'- gGGUUGCCGGCggcGG-CCaucUGcugagccagugcuUUCACCc -3' miRNA: 3'- gCCAACGGCCGa--CCaGGa--AC-------------AAGUGG- -5' |
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11916 | 3' | -54.9 | NC_003278.1 | + | 5471 | 0.67 | 0.559933 |
Target: 5'- aGGUUGUCGGCUGcGgCCUggcucUCGCg -3' miRNA: 3'- gCCAACGGCCGAC-CaGGAaca--AGUGg -5' |
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11916 | 3' | -54.9 | NC_003278.1 | + | 5619 | 0.67 | 0.53779 |
Target: 5'- -cGUUGCCGGC-GGUCUUcUGgccggUCACg -3' miRNA: 3'- gcCAACGGCCGaCCAGGA-ACa----AGUGg -5' |
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11916 | 3' | -54.9 | NC_003278.1 | + | 14136 | 0.67 | 0.52683 |
Target: 5'- uGGaUGCCGaGCUuGUCCUUGa--ACCa -3' miRNA: 3'- gCCaACGGC-CGAcCAGGAACaagUGG- -5' |
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11916 | 3' | -54.9 | NC_003278.1 | + | 21637 | 0.68 | 0.515956 |
Target: 5'- cCGGggacGCCGGCgggGGUCUguugcCACCa -3' miRNA: 3'- -GCCaa--CGGCCGa--CCAGGaacaaGUGG- -5' |
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11916 | 3' | -54.9 | NC_003278.1 | + | 33204 | 0.68 | 0.473437 |
Target: 5'- gCGGUUccGCCGGCUGcGgCCgaGgccaUCGCCg -3' miRNA: 3'- -GCCAA--CGGCCGAC-CaGGaaCa---AGUGG- -5' |
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11916 | 3' | -54.9 | NC_003278.1 | + | 13217 | 0.69 | 0.442734 |
Target: 5'- aGGUUGCCGGC-GGUaCCgaGgaccgaGCCg -3' miRNA: 3'- gCCAACGGCCGaCCA-GGaaCaag---UGG- -5' |
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11916 | 3' | -54.9 | NC_003278.1 | + | 3812 | 0.69 | 0.432751 |
Target: 5'- gCGGagGCCGGCcgGGUCCacGUcgaugcCACCa -3' miRNA: 3'- -GCCaaCGGCCGa-CCAGGaaCAa-----GUGG- -5' |
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11916 | 3' | -54.9 | NC_003278.1 | + | 2812 | 0.7 | 0.394178 |
Target: 5'- aCGG-UGCCGGCUGGgCCg---UC-CCa -3' miRNA: 3'- -GCCaACGGCCGACCaGGaacaAGuGG- -5' |
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11916 | 3' | -54.9 | NC_003278.1 | + | 11868 | 0.7 | 0.384888 |
Target: 5'- ---cUGaCCGGCUGGcugCCUUGggcaUCGCCg -3' miRNA: 3'- gccaAC-GGCCGACCa--GGAACa---AGUGG- -5' |
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11916 | 3' | -54.9 | NC_003278.1 | + | 3307 | 0.73 | 0.256736 |
Target: 5'- uCGGcgGCCGGCUuGcCCUUGUugaagcgcUCGCCa -3' miRNA: 3'- -GCCaaCGGCCGAcCaGGAACA--------AGUGG- -5' |
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11916 | 3' | -54.9 | NC_003278.1 | + | 29559 | 1.13 | 0.000357 |
Target: 5'- uCGGUUGCCGGCUGGUCCUUGUUCACCa -3' miRNA: 3'- -GCCAACGGCCGACCAGGAACAAGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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