Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11916 | 5' | -55.1 | NC_003278.1 | + | 32543 | 0.66 | 0.619805 |
Target: 5'- ----cUGUCGcGGACCcGCgcgCGcCGGCa -3' miRNA: 3'- caugaACAGCaCCUGGuCGa--GC-GCCG- -5' |
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11916 | 5' | -55.1 | NC_003278.1 | + | 8730 | 0.66 | 0.608443 |
Target: 5'- -aGgUUGUCGaguUGGGuuGGCUCG-GGCa -3' miRNA: 3'- caUgAACAGC---ACCUggUCGAGCgCCG- -5' |
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11916 | 5' | -55.1 | NC_003278.1 | + | 28051 | 0.66 | 0.585794 |
Target: 5'- -cGCUUgagaaaGUCGcGGAucgcCCAGCgcuggUCGCGGCg -3' miRNA: 3'- caUGAA------CAGCaCCU----GGUCG-----AGCGCCG- -5' |
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11916 | 5' | -55.1 | NC_003278.1 | + | 32768 | 0.67 | 0.552153 |
Target: 5'- uUGC-UGUCGcGGACCcGCgccacCGUGGCc -3' miRNA: 3'- cAUGaACAGCaCCUGGuCGa----GCGCCG- -5' |
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11916 | 5' | -55.1 | NC_003278.1 | + | 30703 | 0.67 | 0.541063 |
Target: 5'- ----gUGUCG-GGAUUGGuCUUGCGGCu -3' miRNA: 3'- caugaACAGCaCCUGGUC-GAGCGCCG- -5' |
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11916 | 5' | -55.1 | NC_003278.1 | + | 4793 | 0.67 | 0.530048 |
Target: 5'- -----aGUCGUGGAUCAGUugcagauagUCGUGGg -3' miRNA: 3'- caugaaCAGCACCUGGUCG---------AGCGCCg -5' |
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11916 | 5' | -55.1 | NC_003278.1 | + | 25939 | 0.68 | 0.486891 |
Target: 5'- -cGCUguuggucagGUCGgccUGGGCCuggcGCUCGCGGg -3' miRNA: 3'- caUGAa--------CAGC---ACCUGGu---CGAGCGCCg -5' |
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11916 | 5' | -55.1 | NC_003278.1 | + | 8579 | 0.68 | 0.445468 |
Target: 5'- -gGCaUGUCGUGcGGCCAgguGUUgGCGGUg -3' miRNA: 3'- caUGaACAGCAC-CUGGU---CGAgCGCCG- -5' |
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11916 | 5' | -55.1 | NC_003278.1 | + | 31521 | 0.69 | 0.409928 |
Target: 5'- -gGC-UGUCGagUGGGCCAGCUacauggccaaccugGCGGCc -3' miRNA: 3'- caUGaACAGC--ACCUGGUCGAg-------------CGCCG- -5' |
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11916 | 5' | -55.1 | NC_003278.1 | + | 18825 | 0.71 | 0.33341 |
Target: 5'- aGUGCUgagcGUCGUGGuACCaaccucgAGCucgUCGCGGUg -3' miRNA: 3'- -CAUGAa---CAGCACC-UGG-------UCG---AGCGCCG- -5' |
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11916 | 5' | -55.1 | NC_003278.1 | + | 14145 | 0.71 | 0.309812 |
Target: 5'- -aGCUUGUCcuUGaACCAGUcgaUCGCGGCg -3' miRNA: 3'- caUGAACAGc-ACcUGGUCG---AGCGCCG- -5' |
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11916 | 5' | -55.1 | NC_003278.1 | + | 29593 | 1.11 | 0.000438 |
Target: 5'- aGUACUUGUCGUGGACCAGCUCGCGGCc -3' miRNA: 3'- -CAUGAACAGCACCUGGUCGAGCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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