Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11917 | 5' | -61.4 | NC_003278.1 | + | 12811 | 0.66 | 0.329056 |
Target: 5'- cCCGCCUCGcCCUGGa-GUCCgaaccGCUGa -3' miRNA: 3'- -GGUGGAGCaGGACCcgCGGGaa---CGGC- -5' |
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11917 | 5' | -61.4 | NC_003278.1 | + | 26046 | 0.66 | 0.328269 |
Target: 5'- gCCAaCUCGgCCUGGGCcaggcggGCCUgcGCCa -3' miRNA: 3'- -GGUgGAGCaGGACCCG-------CGGGaaCGGc -5' |
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11917 | 5' | -61.4 | NC_003278.1 | + | 9129 | 0.66 | 0.316617 |
Target: 5'- uCCACUggcagCG-CCUGGGCuacgucagcgugcagGCCUUcGCCGa -3' miRNA: 3'- -GGUGGa----GCaGGACCCG---------------CGGGAaCGGC- -5' |
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11917 | 5' | -61.4 | NC_003278.1 | + | 25452 | 0.66 | 0.313563 |
Target: 5'- uCCAUCaguUCGaCCUGGGCGgCCgagucaGCCa -3' miRNA: 3'- -GGUGG---AGCaGGACCCGCgGGaa----CGGc -5' |
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11917 | 5' | -61.4 | NC_003278.1 | + | 22330 | 0.66 | 0.313563 |
Target: 5'- gCUGCCgUUGcCCUGGGCGgCCUgcaCCGu -3' miRNA: 3'- -GGUGG-AGCaGGACCCGCgGGAac-GGC- -5' |
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11917 | 5' | -61.4 | NC_003278.1 | + | 27739 | 0.66 | 0.306026 |
Target: 5'- aCCGCCUCGgucacgCCggcGGuGCGaCCguagUGCCGc -3' miRNA: 3'- -GGUGGAGCa-----GGa--CC-CGCgGGa---ACGGC- -5' |
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11917 | 5' | -61.4 | NC_003278.1 | + | 9006 | 0.66 | 0.29137 |
Target: 5'- aC-CCUCG-CCcGGGCGgCUaUGCCGg -3' miRNA: 3'- gGuGGAGCaGGaCCCGCgGGaACGGC- -5' |
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11917 | 5' | -61.4 | NC_003278.1 | + | 28110 | 0.67 | 0.284251 |
Target: 5'- -uGCgaUGUCCUGGGCcuuGUCgUUGCCGc -3' miRNA: 3'- ggUGgaGCAGGACCCG---CGGgAACGGC- -5' |
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11917 | 5' | -61.4 | NC_003278.1 | + | 24814 | 0.67 | 0.284251 |
Target: 5'- -aACCgugaUCG-CCUGGGCgucGCCCUUGgCGc -3' miRNA: 3'- ggUGG----AGCaGGACCCG---CGGGAACgGC- -5' |
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11917 | 5' | -61.4 | NC_003278.1 | + | 8364 | 0.67 | 0.27727 |
Target: 5'- gCGCCUgGUCugcuaccagcgCUGcGGCGCCCgcuuccGCCGc -3' miRNA: 3'- gGUGGAgCAG-----------GAC-CCGCGGGaa----CGGC- -5' |
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11917 | 5' | -61.4 | NC_003278.1 | + | 5998 | 0.67 | 0.266385 |
Target: 5'- aCCgACCUCaUCCugucgcagaagcgcaUGGGCGgCCUgccGCCGg -3' miRNA: 3'- -GG-UGGAGcAGG---------------ACCCGCgGGAa--CGGC- -5' |
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11917 | 5' | -61.4 | NC_003278.1 | + | 13713 | 0.67 | 0.25071 |
Target: 5'- gUACCUgG-CCUGGGCGUUCUccGUCGa -3' miRNA: 3'- gGUGGAgCaGGACCCGCGGGAa-CGGC- -5' |
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11917 | 5' | -61.4 | NC_003278.1 | + | 13793 | 0.69 | 0.193453 |
Target: 5'- cCUACCUCGUCCacucccacaagGGaaccuugaGCGCCCUgcGCCGc -3' miRNA: 3'- -GGUGGAGCAGGa----------CC--------CGCGGGAa-CGGC- -5' |
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11917 | 5' | -61.4 | NC_003278.1 | + | 28216 | 0.73 | 0.106369 |
Target: 5'- gCCGCCUCGgCCcGGGCGUaCCUgGCCc -3' miRNA: 3'- -GGUGGAGCaGGaCCCGCG-GGAaCGGc -5' |
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11917 | 5' | -61.4 | NC_003278.1 | + | 7995 | 0.73 | 0.095129 |
Target: 5'- -gGCCUCGgCCUGGGCGuaaCCCUU-CCGg -3' miRNA: 3'- ggUGGAGCaGGACCCGC---GGGAAcGGC- -5' |
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11917 | 5' | -61.4 | NC_003278.1 | + | 21232 | 0.77 | 0.052429 |
Target: 5'- uUCGCCggCGUCCUGGGCuaCCUgggcaucgaccUGCCGg -3' miRNA: 3'- -GGUGGa-GCAGGACCCGcgGGA-----------ACGGC- -5' |
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11917 | 5' | -61.4 | NC_003278.1 | + | 16016 | 0.78 | 0.044127 |
Target: 5'- gCCAagUCGgaauacUCCUGGGCGUCCUUGCCu -3' miRNA: 3'- -GGUggAGC------AGGACCCGCGGGAACGGc -5' |
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11917 | 5' | -61.4 | NC_003278.1 | + | 29029 | 1.11 | 0.000114 |
Target: 5'- aCCACCUCGUCCUGGGCGCCCUUGCCGg -3' miRNA: 3'- -GGUGGAGCAGGACCCGCGGGAACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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