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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11920 | 5' | -51.1 | NC_003278.1 | + | 7361 | 0.66 | 0.812396 |
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Target: 5'- cGcCGccGAAGUGGcCGAGCGCUa-- -3' miRNA: 3'- aCaGCuaCUUCGCCuGCUUGCGAagc -5' |
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| 11920 | 5' | -51.1 | NC_003278.1 | + | 7003 | 0.7 | 0.612751 |
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Target: 5'- cGUCGAUGccGUGGugcagcagcGCGAugGCgUCGa -3' miRNA: 3'- aCAGCUACuuCGCC---------UGCUugCGaAGC- -5' |
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| 11920 | 5' | -51.1 | NC_003278.1 | + | 33006 | 0.71 | 0.555283 |
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Target: 5'- gGUCGAUGGAGCGuGGcCGAuccGCGCa--- -3' miRNA: 3'- aCAGCUACUUCGC-CU-GCU---UGCGaagc -5' |
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| 11920 | 5' | -51.1 | NC_003278.1 | + | 19148 | 0.71 | 0.543956 |
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Target: 5'- --cUGAUGGAGUGGcGCGAACGCgccCGg -3' miRNA: 3'- acaGCUACUUCGCC-UGCUUGCGaa-GC- -5' |
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| 11920 | 5' | -51.1 | NC_003278.1 | + | 8902 | 0.72 | 0.467228 |
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Target: 5'- gGUCGAggcccAGGCGGGCGGcgGCGCaUUCGc -3' miRNA: 3'- aCAGCUac---UUCGCCUGCU--UGCG-AAGC- -5' |
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| 11920 | 5' | -51.1 | NC_003278.1 | + | 28786 | 0.77 | 0.277289 |
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Target: 5'- -aUCGAUGAcGCGGcCGAGCGCgUCGc -3' miRNA: 3'- acAGCUACUuCGCCuGCUUGCGaAGC- -5' |
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| 11920 | 5' | -51.1 | NC_003278.1 | + | 26315 | 1.1 | 0.001432 |
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Target: 5'- gUGUCGAUGAAGCGGACGAACGCUUCGa -3' miRNA: 3'- -ACAGCUACUUCGCCUGCUUGCGAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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