Results 1 - 20 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11921 | 3' | -58.3 | NC_003278.1 | + | 20099 | 0.66 | 0.410669 |
Target: 5'- -aGGUccaugUCGCCcGCGGCUgccaGGuuGAGCAu -3' miRNA: 3'- gaCCA-----AGUGGuCGUCGG----UCggCUCGU- -5' |
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11921 | 3' | -58.3 | NC_003278.1 | + | 16825 | 0.66 | 0.410669 |
Target: 5'- -cGGaaacUCACCGGacucCGGCCAGUCGcGCAg -3' miRNA: 3'- gaCCa---AGUGGUC----GUCGGUCGGCuCGU- -5' |
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11921 | 3' | -58.3 | NC_003278.1 | + | 22096 | 0.66 | 0.4078 |
Target: 5'- -gGGUgaucgcgucgUCGCCgcgaccgacgaacuGGCGcGCCGGCUGGGCAc -3' miRNA: 3'- gaCCA----------AGUGG--------------UCGU-CGGUCGGCUCGU- -5' |
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11921 | 3' | -58.3 | NC_003278.1 | + | 22814 | 0.66 | 0.400213 |
Target: 5'- -cGGUgcucgCGCCGGCgcuggcgGGCCuGCCGAuccuGCAg -3' miRNA: 3'- gaCCAa----GUGGUCG-------UCGGuCGGCU----CGU- -5' |
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11921 | 3' | -58.3 | NC_003278.1 | + | 21608 | 0.66 | 0.390857 |
Target: 5'- --cGUUCgACCAGCcgcugcuGGCCAuGCUGGGCGa -3' miRNA: 3'- gacCAAG-UGGUCG-------UCGGU-CGGCUCGU- -5' |
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11921 | 3' | -58.3 | NC_003278.1 | + | 21982 | 0.66 | 0.386233 |
Target: 5'- gUGGcgacaCGCCAGCAGCagccgcaucggugccCAGCCGGcGCGc -3' miRNA: 3'- gACCaa---GUGGUCGUCG---------------GUCGGCU-CGU- -5' |
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11921 | 3' | -58.3 | NC_003278.1 | + | 7674 | 0.66 | 0.38256 |
Target: 5'- -cGGUUUcuACCucGCGGCacacggcaUAGCCGAGCGg -3' miRNA: 3'- gaCCAAG--UGGu-CGUCG--------GUCGGCUCGU- -5' |
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11921 | 3' | -58.3 | NC_003278.1 | + | 26148 | 0.66 | 0.373479 |
Target: 5'- uUGGUUCAgcCCAauacgcuugcGCucAGCCuguAGCCGAGCGc -3' miRNA: 3'- gACCAAGU--GGU----------CG--UCGG---UCGGCUCGU- -5' |
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11921 | 3' | -58.3 | NC_003278.1 | + | 28100 | 0.67 | 0.35576 |
Target: 5'- cCUGGUUCAUCcGCuGCgGGCgCG-GCAa -3' miRNA: 3'- -GACCAAGUGGuCGuCGgUCG-GCuCGU- -5' |
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11921 | 3' | -58.3 | NC_003278.1 | + | 20329 | 0.67 | 0.347126 |
Target: 5'- cCUGGa-CGCCAGC-GUU-GCCGAGCAg -3' miRNA: 3'- -GACCaaGUGGUCGuCGGuCGGCUCGU- -5' |
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11921 | 3' | -58.3 | NC_003278.1 | + | 21467 | 0.67 | 0.347126 |
Target: 5'- -aGGgccucggCGCCGGCcaGGCCGGCCcgcuGGGCGu -3' miRNA: 3'- gaCCaa-----GUGGUCG--UCGGUCGG----CUCGU- -5' |
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11921 | 3' | -58.3 | NC_003278.1 | + | 19491 | 0.67 | 0.338642 |
Target: 5'- -gGGUUgcCGgCGGCGGCCAucuGCUGAGCc -3' miRNA: 3'- gaCCAA--GUgGUCGUCGGU---CGGCUCGu -5' |
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11921 | 3' | -58.3 | NC_003278.1 | + | 23244 | 0.67 | 0.32213 |
Target: 5'- ----cUCGCCAGgGGCCGGCCGGa-- -3' miRNA: 3'- gaccaAGUGGUCgUCGGUCGGCUcgu -5' |
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11921 | 3' | -58.3 | NC_003278.1 | + | 33205 | 0.67 | 0.314102 |
Target: 5'- -cGGUUcCGCCGGCuG-CGGCCGAGg- -3' miRNA: 3'- gaCCAA-GUGGUCGuCgGUCGGCUCgu -5' |
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11921 | 3' | -58.3 | NC_003278.1 | + | 27243 | 0.68 | 0.309359 |
Target: 5'- aUGGggagCACCAGCauuGGCCgcucgaggcccgaggGGCUGAGCu -3' miRNA: 3'- gACCaa--GUGGUCG---UCGG---------------UCGGCUCGu -5' |
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11921 | 3' | -58.3 | NC_003278.1 | + | 11931 | 0.68 | 0.306227 |
Target: 5'- -cGGcgaUGCCcaaGGCAGCCAGCCGgucAGCAg -3' miRNA: 3'- gaCCaa-GUGG---UCGUCGGUCGGC---UCGU- -5' |
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11921 | 3' | -58.3 | NC_003278.1 | + | 2769 | 0.68 | 0.306227 |
Target: 5'- aCUGGcgCACCAGCuGCgCGacGCCaGAGCc -3' miRNA: 3'- -GACCaaGUGGUCGuCG-GU--CGG-CUCGu -5' |
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11921 | 3' | -58.3 | NC_003278.1 | + | 429 | 0.68 | 0.269122 |
Target: 5'- gCUGGUUCACUgaaAGUAcCCAGUaCGGGCGg -3' miRNA: 3'- -GACCAAGUGG---UCGUcGGUCG-GCUCGU- -5' |
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11921 | 3' | -58.3 | NC_003278.1 | + | 9073 | 0.69 | 0.255327 |
Target: 5'- -cGGUgcCGCCGGCauAGCCGcCCGGGCGa -3' miRNA: 3'- gaCCAa-GUGGUCG--UCGGUcGGCUCGU- -5' |
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11921 | 3' | -58.3 | NC_003278.1 | + | 32354 | 0.69 | 0.248649 |
Target: 5'- -gGGUUCGCCuggcacuGguGCgAGCCG-GCAg -3' miRNA: 3'- gaCCAAGUGGu------CguCGgUCGGCuCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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