Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11921 | 5' | -61.1 | NC_003278.1 | + | 29888 | 0.66 | 0.323712 |
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Target: 5'- uCGCGC-GUUGGGcGUGaCCGGCcaGAAGa -3' miRNA: 3'- -GUGCGaCAGCCC-CACgGGCCG--CUUCa -5' |
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| 11921 | 5' | -61.1 | NC_003278.1 | + | 7149 | 0.67 | 0.272131 |
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Target: 5'- -uCGCUGUUGauGUGCCCGGCuGGGa -3' miRNA: 3'- guGCGACAGCccCACGGGCCGcUUCa -5' |
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| 11921 | 5' | -61.1 | NC_003278.1 | + | 5741 | 0.67 | 0.265325 |
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Target: 5'- gGCGCgGUUGGuGGUaGCCgCGGCGcuGGUg -3' miRNA: 3'- gUGCGaCAGCC-CCA-CGG-GCCGCu-UCA- -5' |
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| 11921 | 5' | -61.1 | NC_003278.1 | + | 9088 | 0.69 | 0.196177 |
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Target: 5'- gGCGCUGgugcUCGGcGGUgccgccggcauagccGCCCGGgCGAGGg -3' miRNA: 3'- gUGCGAC----AGCC-CCA---------------CGGGCC-GCUUCa -5' |
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| 11921 | 5' | -61.1 | NC_003278.1 | + | 22281 | 0.73 | 0.094801 |
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Target: 5'- -cCGCcG-CGGGGUGCCguCGGCGAAGa -3' miRNA: 3'- guGCGaCaGCCCCACGG--GCCGCUUCa -5' |
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| 11921 | 5' | -61.1 | NC_003278.1 | + | 10144 | 1.06 | 0.000245 |
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Target: 5'- aCACGCUGUCGGGGUGCCCGGCGAAGUc -3' miRNA: 3'- -GUGCGACAGCCCCACGGGCCGCUUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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