Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11922 | 5' | -56 | NC_003278.1 | + | 29623 | 0.66 | 0.561876 |
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Target: 5'- uCCAGUCcuUUCCGuu-CCUCGAGGCc-- -3' miRNA: 3'- -GGUCAG--AAGGUcucGGAGCUCCGaca -5' |
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| 11922 | 5' | -56 | NC_003278.1 | + | 22544 | 0.66 | 0.507084 |
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Target: 5'- gUCGGUCaggUCCAGGGCgaucagUCGuGGGCUGa -3' miRNA: 3'- -GGUCAGa--AGGUCUCGg-----AGC-UCCGACa -5' |
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| 11922 | 5' | -56 | NC_003278.1 | + | 29697 | 0.67 | 0.485797 |
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Target: 5'- aCCGGcCUcgCCGGuGGCCUCGAGGa--- -3' miRNA: 3'- -GGUCaGAa-GGUC-UCGGAGCUCCgaca -5' |
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| 11922 | 5' | -56 | NC_003278.1 | + | 21454 | 0.67 | 0.464944 |
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Target: 5'- gCCGGcCUggcCCAGGGCCUCGGcgccGGCc-- -3' miRNA: 3'- -GGUCaGAa--GGUCUCGGAGCU----CCGaca -5' |
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| 11922 | 5' | -56 | NC_003278.1 | + | 6723 | 0.68 | 0.434567 |
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Target: 5'- gCCGGUCUUCU-GuGCCUUGAGcGCg-- -3' miRNA: 3'- -GGUCAGAAGGuCuCGGAGCUC-CGaca -5' |
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| 11922 | 5' | -56 | NC_003278.1 | + | 5905 | 0.7 | 0.342272 |
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Target: 5'- aCCAcGUCgaacaCCAGGGCgUCGAGGUucuUGUa -3' miRNA: 3'- -GGU-CAGaa---GGUCUCGgAGCUCCG---ACA- -5' |
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| 11922 | 5' | -56 | NC_003278.1 | + | 8298 | 0.7 | 0.301724 |
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Target: 5'- aCCuuGUCgacCCAGGcgaccGCCUUGAGGCUGa -3' miRNA: 3'- -GGu-CAGaa-GGUCU-----CGGAGCUCCGACa -5' |
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| 11922 | 5' | -56 | NC_003278.1 | + | 10514 | 1.09 | 0.000491 |
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Target: 5'- cCCAGUCUUCCAGAGCCUCGAGGCUGUc -3' miRNA: 3'- -GGUCAGAAGGUCUCGGAGCUCCGACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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