Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11923 | 3' | -53 | NC_003278.1 | + | 29202 | 0.66 | 0.747354 |
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Target: 5'- aGUGCccaGGUCgcggCGGCCAGCGAGuucaaGAAGCg -3' miRNA: 3'- gCACG---CUAG----GCUGGUCGCUUc----CUUUG- -5' |
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| 11923 | 3' | -53 | NC_003278.1 | + | 32161 | 0.66 | 0.725372 |
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Target: 5'- --gGCGGUCUGGcCCAGuCGc-GGAAGCg -3' miRNA: 3'- gcaCGCUAGGCU-GGUC-GCuuCCUUUG- -5' |
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| 11923 | 3' | -53 | NC_003278.1 | + | 8536 | 0.66 | 0.714221 |
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Target: 5'- gCGUGCcaGAUgCUGACCGGUGGGcGGuaGAGCa -3' miRNA: 3'- -GCACG--CUA-GGCUGGUCGCUU-CC--UUUG- -5' |
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| 11923 | 3' | -53 | NC_003278.1 | + | 9052 | 0.66 | 0.714221 |
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Target: 5'- cCGgGCGAgggUUC-ACCAGCGcGGGGAACu -3' miRNA: 3'- -GCaCGCU---AGGcUGGUCGCuUCCUUUG- -5' |
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| 11923 | 3' | -53 | NC_003278.1 | + | 20910 | 0.66 | 0.713101 |
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Target: 5'- -cUGCGAUcaucgggCCGagaauGCCGGCGAGGGugauGGCa -3' miRNA: 3'- gcACGCUA-------GGC-----UGGUCGCUUCCu---UUG- -5' |
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| 11923 | 3' | -53 | NC_003278.1 | + | 32245 | 0.68 | 0.611401 |
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Target: 5'- aCGUGcCGcgCC-ACCAGC--AGGAAGCg -3' miRNA: 3'- -GCAC-GCuaGGcUGGUCGcuUCCUUUG- -5' |
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| 11923 | 3' | -53 | NC_003278.1 | + | 34656 | 0.69 | 0.558922 |
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Target: 5'- aGUgGCGAugccgcugcugagcaUCUGGCUGGCGAAGGuguGGCg -3' miRNA: 3'- gCA-CGCU---------------AGGCUGGUCGCUUCCu--UUG- -5' |
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| 11923 | 3' | -53 | NC_003278.1 | + | 16851 | 0.75 | 0.263297 |
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Target: 5'- gGUGCGG-CCGGCCGguguucuGCGAGcGGAAACu -3' miRNA: 3'- gCACGCUaGGCUGGU-------CGCUU-CCUUUG- -5' |
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| 11923 | 3' | -53 | NC_003278.1 | + | 10791 | 1.09 | 0.001001 |
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Target: 5'- gCGUGCGAUCCGACCAGCGAAGGAAACc -3' miRNA: 3'- -GCACGCUAGGCUGGUCGCUUCCUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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