Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11928 | 3' | -54.3 | NC_003278.1 | + | 22238 | 0.66 | 0.638891 |
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Target: 5'- cCGCGG-CgGAUCGAguuCUCCCUCGa -3' miRNA: 3'- aGUGCUgGgCUAGCUauuGAGGGGGU- -5' |
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| 11928 | 3' | -54.3 | NC_003278.1 | + | 7821 | 0.67 | 0.616209 |
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Target: 5'- cCACGGCCUGGcCGAccACggCCCCAc -3' miRNA: 3'- aGUGCUGGGCUaGCUauUGagGGGGU- -5' |
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| 11928 | 3' | -54.3 | NC_003278.1 | + | 26893 | 0.67 | 0.616209 |
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Target: 5'- cCGCG-CCUGAUggCGGUGGCgacgCCCCUg -3' miRNA: 3'- aGUGCuGGGCUA--GCUAUUGa---GGGGGu -5' |
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| 11928 | 3' | -54.3 | NC_003278.1 | + | 28305 | 0.69 | 0.494488 |
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Target: 5'- cCGCGACCUugaGAUCGGUAgcgcgacaguacGCUUCCCg- -3' miRNA: 3'- aGUGCUGGG---CUAGCUAU------------UGAGGGGgu -5' |
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| 11928 | 3' | -54.3 | NC_003278.1 | + | 18701 | 0.7 | 0.413187 |
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Target: 5'- cCGcCGGCCCGGccggCGGUuucuCUCCCCCu -3' miRNA: 3'- aGU-GCUGGGCUa---GCUAuu--GAGGGGGu -5' |
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| 11928 | 3' | -54.3 | NC_003278.1 | + | 16241 | 1.09 | 0.000748 |
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Target: 5'- gUCACGACCCGAUCGAUAACUCCCCCAg -3' miRNA: 3'- -AGUGCUGGGCUAGCUAUUGAGGGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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