Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11929 | 5' | -54.6 | NC_003278.1 | + | 29651 | 0.66 | 0.684086 |
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Target: 5'- gCGCGGaucUGCUCCGCGaaCAGcGAG-CCg -3' miRNA: 3'- gGUGCC---ACGAGGUGCa-GUCcUUCaGG- -5' |
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| 11929 | 5' | -54.6 | NC_003278.1 | + | 2389 | 0.66 | 0.661737 |
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Target: 5'- uCCGaugcCGGUGCUCgACGgcCGGGGcaUCCu -3' miRNA: 3'- -GGU----GCCACGAGgUGCa-GUCCUucAGG- -5' |
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| 11929 | 5' | -54.6 | NC_003278.1 | + | 19121 | 0.67 | 0.616783 |
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Target: 5'- gCCAUGGcGUuuUCUACGcgaUCAGGGAGgcugCCu -3' miRNA: 3'- -GGUGCCaCG--AGGUGC---AGUCCUUCa---GG- -5' |
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| 11929 | 5' | -54.6 | NC_003278.1 | + | 11817 | 0.67 | 0.583176 |
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Target: 5'- gCCGCGGUGgaCCAUGccgcgCAGcgcGUCCg -3' miRNA: 3'- -GGUGCCACgaGGUGCa----GUCcuuCAGG- -5' |
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| 11929 | 5' | -54.6 | NC_003278.1 | + | 31380 | 0.68 | 0.565392 |
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Target: 5'- gCAUGGUGuCUCUccuuuggggugguugGCGcCAGGGAGUUg -3' miRNA: 3'- gGUGCCAC-GAGG---------------UGCaGUCCUUCAGg -5' |
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| 11929 | 5' | -54.6 | NC_003278.1 | + | 2960 | 0.69 | 0.47511 |
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Target: 5'- cCCGCGGUGUcgCC-CGUCucGGcGggGUCa -3' miRNA: 3'- -GGUGCCACGa-GGuGCAG--UC-CuuCAGg -5' |
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| 11929 | 5' | -54.6 | NC_003278.1 | + | 18221 | 0.7 | 0.405703 |
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Target: 5'- aCgGCGGUaGCUaCCaggGCGUCGuGAAGUCCu -3' miRNA: 3'- -GgUGCCA-CGA-GG---UGCAGUcCUUCAGG- -5' |
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| 11929 | 5' | -54.6 | NC_003278.1 | + | 17565 | 1.15 | 0.000299 |
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Target: 5'- aCCACGGUGCUCCACGUCAGGAAGUCCg -3' miRNA: 3'- -GGUGCCACGAGGUGCAGUCCUUCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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