Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1193 | 3' | -43.2 | NC_001132.2 | + | 2057 | 0.75 | 0.995572 |
Target: 5'- gGUGCGUcgAgGgaGACGCCGUGCGu -3' miRNA: 3'- gCAUGUAuaUgCauUUGUGGCAUGCu -5' |
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1193 | 3' | -43.2 | NC_001132.2 | + | 2771 | 0.78 | 0.979733 |
Target: 5'- gCGUGCGUAUACGggGGCGgugccCCGUAUGGc -3' miRNA: 3'- -GCAUGUAUAUGCauUUGU-----GGCAUGCU- -5' |
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1193 | 3' | -43.2 | NC_001132.2 | + | 12517 | 0.71 | 0.999948 |
Target: 5'- aCGUACA--UACGUAAacGCGCCauagGCGAu -3' miRNA: 3'- -GCAUGUauAUGCAUU--UGUGGca--UGCU- -5' |
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1193 | 3' | -43.2 | NC_001132.2 | + | 15799 | 0.69 | 0.999998 |
Target: 5'- cCGUACGcgGUGgGUAuaAACACuCGUACGu -3' miRNA: 3'- -GCAUGUa-UAUgCAU--UUGUG-GCAUGCu -5' |
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1193 | 3' | -43.2 | NC_001132.2 | + | 28270 | 0.68 | 0.999999 |
Target: 5'- aCGUugGCGUGUACGcGAAUAUCGUGuuUGAa -3' miRNA: 3'- -GCA--UGUAUAUGCaUUUGUGGCAU--GCU- -5' |
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1193 | 3' | -43.2 | NC_001132.2 | + | 34750 | 0.69 | 0.999995 |
Target: 5'- --gGCGUGUACGgaGACACCG-ACu- -3' miRNA: 3'- gcaUGUAUAUGCauUUGUGGCaUGcu -5' |
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1193 | 3' | -43.2 | NC_001132.2 | + | 42534 | 0.69 | 0.999995 |
Target: 5'- aCGUGCGg--ACGUu--CGCCGUACc- -3' miRNA: 3'- -GCAUGUauaUGCAuuuGUGGCAUGcu -5' |
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1193 | 3' | -43.2 | NC_001132.2 | + | 45684 | 0.66 | 1 |
Target: 5'- uCGUACAcAUGCGUc-GCGuuCCGUACa- -3' miRNA: 3'- -GCAUGUaUAUGCAuuUGU--GGCAUGcu -5' |
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1193 | 3' | -43.2 | NC_001132.2 | + | 46187 | 0.67 | 1 |
Target: 5'- gCGUAUGUAUGCGccGACGCCuuuuuGUGgGAu -3' miRNA: 3'- -GCAUGUAUAUGCauUUGUGG-----CAUgCU- -5' |
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1193 | 3' | -43.2 | NC_001132.2 | + | 47848 | 0.72 | 0.999737 |
Target: 5'- gCG-ACGUc--CGUAGACGCCGUACGc -3' miRNA: 3'- -GCaUGUAuauGCAUUUGUGGCAUGCu -5' |
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1193 | 3' | -43.2 | NC_001132.2 | + | 70018 | 0.66 | 1 |
Target: 5'- uGUACA---ACGUAGACGgCGUAUc- -3' miRNA: 3'- gCAUGUauaUGCAUUUGUgGCAUGcu -5' |
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1193 | 3' | -43.2 | NC_001132.2 | + | 81465 | 0.66 | 1 |
Target: 5'- aCGUACGUcuaACGUuAAC-UCGUGCGGu -3' miRNA: 3'- -GCAUGUAua-UGCAuUUGuGGCAUGCU- -5' |
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1193 | 3' | -43.2 | NC_001132.2 | + | 88155 | 0.66 | 1 |
Target: 5'- uGUACGUGUACauuaAAACuCCGUuCGAu -3' miRNA: 3'- gCAUGUAUAUGca--UUUGuGGCAuGCU- -5' |
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1193 | 3' | -43.2 | NC_001132.2 | + | 96258 | 0.69 | 0.999993 |
Target: 5'- aCGaGCGuUAUACuu-AACGCCGUACGGg -3' miRNA: 3'- -GCaUGU-AUAUGcauUUGUGGCAUGCU- -5' |
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1193 | 3' | -43.2 | NC_001132.2 | + | 107086 | 0.72 | 0.999795 |
Target: 5'- --cACGUAUcCGUcGAACGCUGUGCGGa -3' miRNA: 3'- gcaUGUAUAuGCA-UUUGUGGCAUGCU- -5' |
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1193 | 3' | -43.2 | NC_001132.2 | + | 112208 | 0.8 | 0.94909 |
Target: 5'- gGUACGUAUGCGUAuGACAUUGUuugGCGAa -3' miRNA: 3'- gCAUGUAUAUGCAU-UUGUGGCA---UGCU- -5' |
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1193 | 3' | -43.2 | NC_001132.2 | + | 129759 | 0.71 | 0.999908 |
Target: 5'- aUGUAgAaagGCGUGGAC-CCGUACGGa -3' miRNA: 3'- -GCAUgUauaUGCAUUUGuGGCAUGCU- -5' |
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1193 | 3' | -43.2 | NC_001132.2 | + | 133204 | 0.66 | 1 |
Target: 5'- aGUA---GUAUGUAgacGACGCCGUccGCGAa -3' miRNA: 3'- gCAUguaUAUGCAU---UUGUGGCA--UGCU- -5' |
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1193 | 3' | -43.2 | NC_001132.2 | + | 141734 | 0.68 | 0.999998 |
Target: 5'- ---cUAUGUGCGUGguuuAACGCgGUGCGGa -3' miRNA: 3'- gcauGUAUAUGCAU----UUGUGgCAUGCU- -5' |
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1193 | 3' | -43.2 | NC_001132.2 | + | 148269 | 0.67 | 1 |
Target: 5'- aCGUuuACGUA-ACGUAGACGggUCGUGCGc -3' miRNA: 3'- -GCA--UGUAUaUGCAUUUGU--GGCAUGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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