Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11930 | 3' | -62 | NC_003278.1 | + | 31653 | 0.66 | 0.281828 |
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Target: 5'- -aCCGGGCgCGuCCGcGGGUCgGCaacgUCGAg -3' miRNA: 3'- uaGGUCCG-GC-GGCuCCCAGgUG----AGCU- -5' |
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| 11930 | 3' | -62 | NC_003278.1 | + | 6262 | 0.66 | 0.274838 |
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Target: 5'- -aCCAGGCaGCCGAGGccguaGUCC--UCGAc -3' miRNA: 3'- uaGGUCCGgCGGCUCC-----CAGGugAGCU- -5' |
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| 11930 | 3' | -62 | NC_003278.1 | + | 4867 | 0.67 | 0.212423 |
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Target: 5'- gGUCUugcGGCUGgCGAGGGUCCcguuCUUGGc -3' miRNA: 3'- -UAGGu--CCGGCgGCUCCCAGGu---GAGCU- -5' |
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| 11930 | 3' | -62 | NC_003278.1 | + | 31786 | 0.68 | 0.19467 |
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Target: 5'- -gCCAGGCgCucgaagucgcggagGCCGGccGGGUCCACgUCGAu -3' miRNA: 3'- uaGGUCCG-G--------------CGGCU--CCCAGGUG-AGCU- -5' |
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| 11930 | 3' | -62 | NC_003278.1 | + | 5695 | 0.68 | 0.191075 |
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Target: 5'- -aUCAGGCgGUCGAGGGcCUggcGCUUGAg -3' miRNA: 3'- uaGGUCCGgCGGCUCCCaGG---UGAGCU- -5' |
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| 11930 | 3' | -62 | NC_003278.1 | + | 26856 | 0.69 | 0.171634 |
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Target: 5'- gGUCgAGGuuGUCGAguuGGGUUgGCUCGGg -3' miRNA: 3'- -UAGgUCCggCGGCU---CCCAGgUGAGCU- -5' |
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| 11930 | 3' | -62 | NC_003278.1 | + | 13945 | 0.69 | 0.167057 |
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Target: 5'- -gCCGGggacGCCGgCGGGGGUcuguugccaCCACUCGAg -3' miRNA: 3'- uaGGUC----CGGCgGCUCCCA---------GGUGAGCU- -5' |
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| 11930 | 3' | -62 | NC_003278.1 | + | 8814 | 0.7 | 0.149829 |
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Target: 5'- -gUCAGcGCCGCCGAGGGcggCCAgggucUUCGGg -3' miRNA: 3'- uaGGUC-CGGCGGCUCCCa--GGU-----GAGCU- -5' |
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| 11930 | 3' | -62 | NC_003278.1 | + | 18417 | 1.06 | 0.000181 |
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Target: 5'- gAUCCAGGCCGCCGAGGGUCCACUCGAa -3' miRNA: 3'- -UAGGUCCGGCGGCUCCCAGGUGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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