Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11933 | 3' | -53.9 | NC_003278.1 | + | 2577 | 0.66 | 0.68296 |
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Target: 5'- uGCGgccggCGGUauaggugaacuggCGCCCACcGGCCGUGa- -3' miRNA: 3'- -CGCaa---GCUA-------------GUGGGUGuCCGGCAUcu -5' |
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| 11933 | 3' | -53.9 | NC_003278.1 | + | 10125 | 0.66 | 0.67279 |
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Target: 5'- gGCGaagUCGGugaggaUCACCUGCAGGUCaUAGGc -3' miRNA: 3'- -CGCa--AGCU------AGUGGGUGUCCGGcAUCU- -5' |
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| 11933 | 3' | -53.9 | NC_003278.1 | + | 14122 | 0.67 | 0.638698 |
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Target: 5'- gGUGUcgccgUCGAUCGuCgC-CAGGCCGUGGc -3' miRNA: 3'- -CGCA-----AGCUAGU-GgGuGUCCGGCAUCu -5' |
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| 11933 | 3' | -53.9 | NC_003278.1 | + | 32608 | 0.69 | 0.526176 |
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Target: 5'- gGCGUUUGccggCGCgCGCGGGCCGcgacagAGAa -3' miRNA: 3'- -CGCAAGCua--GUGgGUGUCCGGCa-----UCU- -5' |
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| 11933 | 3' | -53.9 | NC_003278.1 | + | 21387 | 0.69 | 0.483096 |
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Target: 5'- cGCGcUUGAUCugCCcuaagccggcgaGCAGGCCGUu-- -3' miRNA: 3'- -CGCaAGCUAGugGG------------UGUCCGGCAucu -5' |
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| 11933 | 3' | -53.9 | NC_003278.1 | + | 1588 | 0.7 | 0.451931 |
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Target: 5'- uGCG---GAUagACCCGCAGGCCGgcGAg -3' miRNA: 3'- -CGCaagCUAg-UGGGUGUCCGGCauCU- -5' |
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| 11933 | 3' | -53.9 | NC_003278.1 | + | 28728 | 0.7 | 0.451931 |
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Target: 5'- cGCGccUCGGUCACCCACc-GCCGg--- -3' miRNA: 3'- -CGCa-AGCUAGUGGGUGucCGGCaucu -5' |
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| 11933 | 3' | -53.9 | NC_003278.1 | + | 21036 | 0.71 | 0.412155 |
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Target: 5'- cGCGcagggCGGUCACCaGCGGGCCGa--- -3' miRNA: 3'- -CGCaa---GCUAGUGGgUGUCCGGCaucu -5' |
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| 11933 | 3' | -53.9 | NC_003278.1 | + | 3679 | 0.72 | 0.339466 |
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Target: 5'- aGCGcgUCGAUCagcGCCuCGCGGGCgaaGUAGAa -3' miRNA: 3'- -CGCa-AGCUAG---UGG-GUGUCCGg--CAUCU- -5' |
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| 11933 | 3' | -53.9 | NC_003278.1 | + | 22234 | 1.11 | 0.000584 |
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Target: 5'- cGCGUUCGAUCACCCACAGGCCGUAGAu -3' miRNA: 3'- -CGCAAGCUAGUGGGUGUCCGGCAUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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