miRNA: 3'- aCGGga------GCUGGuACCCGg--UC-CGGCUG- -5'
miRNA: 3'- aCGGGagCUGGU------ACCcGGuCCGGCUG- -5'
miRNA: 3'- aCGGGA-GC---------------UGGUA-CCCGGuCCGGCuG- -5'
miRNA: 3'- aCGggAGCUGGUaCCCGGUCCggCUG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11933 | 5' | -62.6 | NC_003278.1 | + | 21627 | 0.66 | 0.24953 |
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Target: 5'- gGCCaugcugggCGACC-UGGGCaagAGcGCCGGCg -3' miRNA: 3'- aCGGga------GCUGGuACCCGg--UC-CGGCUG- -5' |
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| 11933 | 5' | -62.6 | NC_003278.1 | + | 18967 | 0.66 | 0.24953 |
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Target: 5'- aGCCCgaGGCCAccggcaUGGaCCcGGCCGACc -3' miRNA: 3'- aCGGGagCUGGU------ACCcGGuCCGGCUG- -5' |
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| 11933 | 5' | -62.6 | NC_003278.1 | + | 20991 | 0.66 | 0.252099 |
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Target: 5'- cGCCCUgCGcgucgugagcaucgcGCUGUgGGGCCuGGCCGcCa -3' miRNA: 3'- aCGGGA-GC---------------UGGUA-CCCGGuCCGGCuG- -5' |
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| 11933 | 5' | -62.6 | NC_003278.1 | + | 27518 | 0.66 | 0.255991 |
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Target: 5'- gGCa-UCGACUG-GGGCCAGGgaGACg -3' miRNA: 3'- aCGggAGCUGGUaCCCGGUCCggCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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