Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11935 | 5' | -51.4 | NC_003278.1 | + | 18146 | 0.66 | 0.820124 |
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Target: 5'- aCGCGGcUGGCGaagUCGAcgAGAUAgCUgUCGGu -3' miRNA: 3'- -GCGCU-ACCGC---AGCU--UCUAUgGA-AGCU- -5' |
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| 11935 | 5' | -51.4 | NC_003278.1 | + | 13161 | 0.67 | 0.790114 |
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Target: 5'- gGCGcgGGCGUgGAAGAUguaGCUguuacgCGGu -3' miRNA: 3'- gCGCuaCCGCAgCUUCUA---UGGaa----GCU- -5' |
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| 11935 | 5' | -51.4 | NC_003278.1 | + | 14613 | 0.68 | 0.725549 |
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Target: 5'- --gGGUGGCGUaGAcGAUGCCgucgUCGAu -3' miRNA: 3'- gcgCUACCGCAgCUuCUAUGGa---AGCU- -5' |
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| 11935 | 5' | -51.4 | NC_003278.1 | + | 2987 | 0.68 | 0.725549 |
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Target: 5'- gGUGGUGGCGccucCGgcGGUACCgggCGGc -3' miRNA: 3'- gCGCUACCGCa---GCuuCUAUGGaa-GCU- -5' |
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| 11935 | 5' | -51.4 | NC_003278.1 | + | 15389 | 0.7 | 0.622632 |
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Target: 5'- uGCGuUGgauGCGUCGAAGAUugCgaccggUCGAc -3' miRNA: 3'- gCGCuAC---CGCAGCUUCUAugGa-----AGCU- -5' |
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| 11935 | 5' | -51.4 | NC_003278.1 | + | 13128 | 0.7 | 0.599558 |
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Target: 5'- cCGaCGGUcGCGUCGgcGAUGCCU-CGGc -3' miRNA: 3'- -GC-GCUAcCGCAGCuuCUAUGGAaGCU- -5' |
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| 11935 | 5' | -51.4 | NC_003278.1 | + | 17110 | 0.74 | 0.386293 |
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Target: 5'- gGCGGUGGCGUCGgcG---UCUUCGGc -3' miRNA: 3'- gCGCUACCGCAGCuuCuauGGAAGCU- -5' |
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| 11935 | 5' | -51.4 | NC_003278.1 | + | 35252 | 0.75 | 0.34987 |
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Target: 5'- gGgGAUGGUGaaUUGAGGAgcuUACCUUCGAg -3' miRNA: 3'- gCgCUACCGC--AGCUUCU---AUGGAAGCU- -5' |
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| 11935 | 5' | -51.4 | NC_003278.1 | + | 28484 | 0.79 | 0.194039 |
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Target: 5'- aGCuGUGGauCGUCGAAGGUAUCUUCGAc -3' miRNA: 3'- gCGcUACC--GCAGCUUCUAUGGAAGCU- -5' |
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| 11935 | 5' | -51.4 | NC_003278.1 | + | 28557 | 1.09 | 0.001554 |
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Target: 5'- gCGCGAUGGCGUCGAAGAUACCUUCGAc -3' miRNA: 3'- -GCGCUACCGCAGCUUCUAUGGAAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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