Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11937 | 3' | -55.4 | NC_003278.1 | + | 7105 | 0.66 | 0.607153 |
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Target: 5'- uCCgCUCAUGcUGCuGCucgCUUGAGGUCg -3' miRNA: 3'- -GG-GAGUACuGCG-CGccaGAACUCCAG- -5' |
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| 11937 | 3' | -55.4 | NC_003278.1 | + | 28826 | 0.67 | 0.507805 |
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Target: 5'- aCCUCAaGAC-CGCGcGUCaUGAGGg- -3' miRNA: 3'- gGGAGUaCUGcGCGC-CAGaACUCCag -5' |
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| 11937 | 3' | -55.4 | NC_003278.1 | + | 14080 | 0.7 | 0.3877 |
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Target: 5'- gUCCggCGUGAUGCGCaGGUCcagGcGGGUCa -3' miRNA: 3'- -GGGa-GUACUGCGCG-CCAGaa-C-UCCAG- -5' |
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| 11937 | 3' | -55.4 | NC_003278.1 | + | 34201 | 0.7 | 0.3877 |
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Target: 5'- aCCg-AUG-CGCGCGGUUUcgguaccgaggaUGAGGUCg -3' miRNA: 3'- gGGagUACuGCGCGCCAGA------------ACUCCAG- -5' |
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| 11937 | 3' | -55.4 | NC_003278.1 | + | 8020 | 0.7 | 0.378556 |
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Target: 5'- gCCggcCGUGGCG-GUGGUggUGAGGUCa -3' miRNA: 3'- gGGa--GUACUGCgCGCCAgaACUCCAG- -5' |
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| 11937 | 3' | -55.4 | NC_003278.1 | + | 28885 | 1.11 | 0.000422 |
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Target: 5'- gCCCUCAUGACGCGCGGUCUUGAGGUCu -3' miRNA: 3'- -GGGAGUACUGCGCGCCAGAACUCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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