Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11938 | 3' | -58.1 | NC_003278.1 | + | 6478 | 0.66 | 0.380341 |
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Target: 5'- gCGUGCUgcaggCCGGCaagGgcGCCCAGGa-- -3' miRNA: 3'- -GUACGAg----GGCCGa--CauCGGGUCCauu -5' |
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| 11938 | 3' | -58.1 | NC_003278.1 | + | 35090 | 0.66 | 0.371218 |
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Target: 5'- aCAUGaaaUCCGGCUGUuGCCCuGGg-- -3' miRNA: 3'- -GUACga-GGGCCGACAuCGGGuCCauu -5' |
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| 11938 | 3' | -58.1 | NC_003278.1 | + | 27090 | 0.68 | 0.295998 |
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Target: 5'- --cGCaUCCGGCUGUGGCagCAGGg-- -3' miRNA: 3'- guaCGaGGGCCGACAUCGg-GUCCauu -5' |
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| 11938 | 3' | -58.1 | NC_003278.1 | + | 19528 | 0.72 | 0.145049 |
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Target: 5'- gAUGg-CCCGGUUGUAGUCgCGGGUGAg -3' miRNA: 3'- gUACgaGGGCCGACAUCGG-GUCCAUU- -5' |
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| 11938 | 3' | -58.1 | NC_003278.1 | + | 5188 | 0.75 | 0.09373 |
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Target: 5'- -cUGCUgUUGGCUGUGGuCCCGGGUGc -3' miRNA: 3'- guACGAgGGCCGACAUC-GGGUCCAUu -5' |
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| 11938 | 3' | -58.1 | NC_003278.1 | + | 29406 | 1.05 | 0.000407 |
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Target: 5'- gCAUGCUCCCGGCUGUAGCCCAGGUAAu -3' miRNA: 3'- -GUACGAGGGCCGACAUCGGGUCCAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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