Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11944 | 3' | -58.5 | NC_003278.1 | + | 26773 | 0.66 | 0.378031 |
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Target: 5'- cGGCaUGUUCGuGcagAUGcCCGAGCCAAc -3' miRNA: 3'- uCCG-ACGAGCuCca-UAC-GGCUCGGUU- -5' |
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| 11944 | 3' | -58.5 | NC_003278.1 | + | 22365 | 0.68 | 0.272159 |
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Target: 5'- cAGGUUGC-CGGcGGUAccgagGaCCGAGCCGAg -3' miRNA: 3'- -UCCGACGaGCU-CCAUa----C-GGCUCGGUU- -5' |
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| 11944 | 3' | -58.5 | NC_003278.1 | + | 9601 | 0.68 | 0.265085 |
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Target: 5'- aGGGCgGcCUCGAGGgccgugaugGUGCCG-GCCu- -3' miRNA: 3'- -UCCGaC-GAGCUCCa--------UACGGCuCGGuu -5' |
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| 11944 | 3' | -58.5 | NC_003278.1 | + | 10639 | 0.68 | 0.25816 |
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Target: 5'- aGGcGCUGCgUCGGGGUugcGCUGuGCCAu -3' miRNA: 3'- -UC-CGACG-AGCUCCAua-CGGCuCGGUu -5' |
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| 11944 | 3' | -58.5 | NC_003278.1 | + | 19650 | 0.68 | 0.251383 |
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Target: 5'- cAGGUcgcgcucaUGCUggcCGAGGUugcggGUGCUGAGCCGg -3' miRNA: 3'- -UCCG--------ACGA---GCUCCA-----UACGGCUCGGUu -5' |
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| 11944 | 3' | -58.5 | NC_003278.1 | + | 27226 | 0.69 | 0.238271 |
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Target: 5'- uGGCcGCUCGAGGcccgagggGCUGAGCUg- -3' miRNA: 3'- uCCGaCGAGCUCCaua-----CGGCUCGGuu -5' |
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| 11944 | 3' | -58.5 | NC_003278.1 | + | 30084 | 0.69 | 0.21968 |
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Target: 5'- uGGGCgUGCUCGAGGUcagccacuuccUGCUGAugGCCAu -3' miRNA: 3'- -UCCG-ACGAGCUCCAu----------ACGGCU--CGGUu -5' |
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| 11944 | 3' | -58.5 | NC_003278.1 | + | 2095 | 1.06 | 0.000321 |
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Target: 5'- gAGGCUGCUCGAGGUAUGCCGAGCCAAa -3' miRNA: 3'- -UCCGACGAGCUCCAUACGGCUCGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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