Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11944 | 5' | -53.9 | NC_003278.1 | + | 18810 | 0.66 | 0.645909 |
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Target: 5'- aGGUCagcGGGAGu-CUGUUCGGuuuucauggGCGUCg -3' miRNA: 3'- -UCAGgu-CCCUCuuGACGAGCU---------UGCAG- -5' |
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| 11944 | 5' | -53.9 | NC_003278.1 | + | 14064 | 0.67 | 0.575929 |
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Target: 5'- aGGUCCAGGcGGGucaggugucggcuGACUGggCGcACGUCg -3' miRNA: 3'- -UCAGGUCC-CUC-------------UUGACgaGCuUGCAG- -5' |
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| 11944 | 5' | -53.9 | NC_003278.1 | + | 5932 | 0.68 | 0.55441 |
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Target: 5'- cGGUgCCAGGGAucGAucagGCUGCU-GAccACGUCg -3' miRNA: 3'- -UCA-GGUCCCU--CU----UGACGAgCU--UGCAG- -5' |
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| 11944 | 5' | -53.9 | NC_003278.1 | + | 2082 | 0.7 | 0.440232 |
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Target: 5'- --cCCAGGcGAGAacgagGCUGCUCGAgguaugccgagccaaAUGUCa -3' miRNA: 3'- ucaGGUCC-CUCU-----UGACGAGCU---------------UGCAG- -5' |
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| 11944 | 5' | -53.9 | NC_003278.1 | + | 18176 | 0.72 | 0.301222 |
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Target: 5'- cGUCCGGGGAGcGCUGggCGucagGCGUUg -3' miRNA: 3'- uCAGGUCCCUCuUGACgaGCu---UGCAG- -5' |
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| 11944 | 5' | -53.9 | NC_003278.1 | + | 2130 | 1.1 | 0.000588 |
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Target: 5'- cAGUCCAGGGAGAACUGCUCGAACGUCg -3' miRNA: 3'- -UCAGGUCCCUCUUGACGAGCUUGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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