Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11947 | 5' | -56.2 | NC_003278.1 | + | 32722 | 0.66 | 0.587437 |
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Target: 5'- aCUCUGGCCGCGGGgaUCGGCga-CGg -3' miRNA: 3'- gGAGGCUGGUGUCCagGGUCGauaGC- -5' |
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| 11947 | 5' | -56.2 | NC_003278.1 | + | 26870 | 0.66 | 0.576352 |
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Target: 5'- gCCcCUGGCgUACAGGUCgaGGUUGUCGa -3' miRNA: 3'- -GGaGGCUG-GUGUCCAGggUCGAUAGC- -5' |
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| 11947 | 5' | -56.2 | NC_003278.1 | + | 32555 | 0.66 | 0.565315 |
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Target: 5'- gCUgCGACCgGCGauGGUgCCCGuGCUGUCGu -3' miRNA: 3'- gGAgGCUGG-UGU--CCA-GGGU-CGAUAGC- -5' |
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| 11947 | 5' | -56.2 | NC_003278.1 | + | 29755 | 0.73 | 0.218795 |
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Target: 5'- gCCUgCG-CCGCuGGcugaCCCAGCUGUCGa -3' miRNA: 3'- -GGAgGCuGGUGuCCa---GGGUCGAUAGC- -5' |
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| 11947 | 5' | -56.2 | NC_003278.1 | + | 13799 | 0.76 | 0.144861 |
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Target: 5'- gCUCCGGCCAgGugcuGUCCCAGCgAUCGa -3' miRNA: 3'- gGAGGCUGGUgUc---CAGGGUCGaUAGC- -5' |
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| 11947 | 5' | -56.2 | NC_003278.1 | + | 3043 | 1.11 | 0.000392 |
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Target: 5'- uCCUCCGACCACAGGUCCCAGCUAUCGa -3' miRNA: 3'- -GGAGGCUGGUGUCCAGGGUCGAUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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