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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11948 | 5' | -54.1 | NC_003278.1 | + | 18064 | 0.69 | 0.508276 |
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Target: 5'- aGUCGUAggUGAuGCGCAGCUUGcCGGc -3' miRNA: 3'- -CAGCAUgaGCUcUGCGUCGAGCaGCU- -5' |
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| 11948 | 5' | -54.1 | NC_003278.1 | + | 32174 | 0.69 | 0.496463 |
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Target: 5'- aGUCGcggaagcgcuuccUGCUgGuGGCGCGGCaCGUCGAg -3' miRNA: 3'- -CAGC-------------AUGAgCuCUGCGUCGaGCAGCU- -5' |
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| 11948 | 5' | -54.1 | NC_003278.1 | + | 13961 | 0.72 | 0.342699 |
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Target: 5'- -cCGUGCUCGAGccgaGCGCcuGCUCGaCGAc -3' miRNA: 3'- caGCAUGAGCUC----UGCGu-CGAGCaGCU- -5' |
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| 11948 | 5' | -54.1 | NC_003278.1 | + | 30325 | 0.74 | 0.247388 |
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Target: 5'- --gGUGCUCGgacagcggcaccaccAGGCGCAGCgCGUCGAc -3' miRNA: 3'- cagCAUGAGC---------------UCUGCGUCGaGCAGCU- -5' |
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| 11948 | 5' | -54.1 | NC_003278.1 | + | 3158 | 1.09 | 0.000812 |
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Target: 5'- cGUCGUACUCGAGACGCAGCUCGUCGAu -3' miRNA: 3'- -CAGCAUGAGCUCUGCGUCGAGCAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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