Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11949 | 3' | -53.6 | NC_003278.1 | + | 4122 | 0.66 | 0.682192 |
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Target: 5'- gACGGAUGGGAc---CGGgcCGaCAGCGUa -3' miRNA: 3'- -UGCCUACCCUuaaaGCU--GCcGUCGCA- -5' |
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| 11949 | 3' | -53.6 | NC_003278.1 | + | 27636 | 0.66 | 0.65933 |
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Target: 5'- aGCGGGccGGGA---UCGggaaGCGGCAGUGa -3' miRNA: 3'- -UGCCUa-CCCUuaaAGC----UGCCGUCGCa -5' |
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| 11949 | 3' | -53.6 | NC_003278.1 | + | 23048 | 0.67 | 0.631757 |
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Target: 5'- gGCGGGUGaGGuugauguGCGGCAGCGc -3' miRNA: 3'- -UGCCUAC-CCuuaaagcUGCCGUCGCa -5' |
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| 11949 | 3' | -53.6 | NC_003278.1 | + | 16109 | 0.73 | 0.302741 |
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Target: 5'- cUGGGUGGGAAaUUCGAUGGCuucccaGGCa- -3' miRNA: 3'- uGCCUACCCUUaAAGCUGCCG------UCGca -5' |
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| 11949 | 3' | -53.6 | NC_003278.1 | + | 19499 | 0.79 | 0.113746 |
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Target: 5'- cGCGGGUGGGGuugcCGGCGGCGGCc- -3' miRNA: 3'- -UGCCUACCCUuaaaGCUGCCGUCGca -5' |
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| 11949 | 3' | -53.6 | NC_003278.1 | + | 4195 | 1.07 | 0.001258 |
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Target: 5'- cACGGAUGGGAAUUUCGACGGCAGCGUu -3' miRNA: 3'- -UGCCUACCCUUAAAGCUGCCGUCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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