Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11958 | 5' | -52.8 | NC_003278.1 | + | 10569 | 0.66 | 0.730099 |
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Target: 5'- cGCGUGau-GGCacaGCGcaaccccgacggcAGCGCCUAUGAGc -3' miRNA: 3'- -CGUACcuuCCG---CGC-------------UUGCGGAUACUC- -5' |
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| 11958 | 5' | -52.8 | NC_003278.1 | + | 26990 | 0.67 | 0.674522 |
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Target: 5'- uGCAccGGAgggucaAGGCGUGAGCGUCUAc--- -3' miRNA: 3'- -CGUa-CCU------UCCGCGCUUGCGGAUacuc -5' |
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| 11958 | 5' | -52.8 | NC_003278.1 | + | 21276 | 0.67 | 0.674522 |
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Target: 5'- cGCcgGcGAAGGCGCGGuacaucAgGCCgaGUGGGu -3' miRNA: 3'- -CGuaC-CUUCCGCGCU------UgCGGa-UACUC- -5' |
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| 11958 | 5' | -52.8 | NC_003278.1 | + | 19392 | 0.69 | 0.547918 |
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Target: 5'- ---cGGAAGGCGCGcACGUCgcucugcUGAGc -3' miRNA: 3'- cguaCCUUCCGCGCuUGCGGau-----ACUC- -5' |
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| 11958 | 5' | -52.8 | NC_003278.1 | + | 19150 | 0.7 | 0.492498 |
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Target: 5'- -gAUGGAGuGGCGCGAacGCGCCcggguacgGAGu -3' miRNA: 3'- cgUACCUU-CCGCGCU--UGCGGaua-----CUC- -5' |
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| 11958 | 5' | -52.8 | NC_003278.1 | + | 6336 | 1.1 | 0.000829 |
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Target: 5'- gGCAUGGAAGGCGCGAACGCCUAUGAGc -3' miRNA: 3'- -CGUACCUUCCGCGCUUGCGGAUACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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