Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11960 | 3' | -57.3 | NC_003278.1 | + | 29387 | 0.66 | 0.462974 |
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Target: 5'- cCAGGuaauCGA--UGGUgGCCACCGUCu- -3' miRNA: 3'- -GUCCu---GCUccACCAgUGGUGGCAGua -5' |
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| 11960 | 3' | -57.3 | NC_003278.1 | + | 14300 | 0.66 | 0.461953 |
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Target: 5'- gGGGAUGGGGUcagccgucucgccGGgcgCGCCGCCGgCGUc -3' miRNA: 3'- gUCCUGCUCCA-------------CCa--GUGGUGGCaGUA- -5' |
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| 11960 | 3' | -57.3 | NC_003278.1 | + | 4291 | 0.67 | 0.428953 |
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Target: 5'- aGGGGCGAgGGUGGgcggcagcaucugCGCCaugaacgcuGCCGUCGa -3' miRNA: 3'- gUCCUGCU-CCACCa------------GUGG---------UGGCAGUa -5' |
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| 11960 | 3' | -57.3 | NC_003278.1 | + | 21019 | 0.67 | 0.424075 |
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Target: 5'- uGGGGCcuggccgccaacccGGUGGUCcuggcaauuGCCGCCGUCGUg -3' miRNA: 3'- gUCCUGcu------------CCACCAG---------UGGUGGCAGUA- -5' |
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| 11960 | 3' | -57.3 | NC_003278.1 | + | 20579 | 0.67 | 0.423103 |
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Target: 5'- gAGGGCGAGGccgcgagGGUCGCCGCgacccaGUUg- -3' miRNA: 3'- gUCCUGCUCCa------CCAGUGGUGg-----CAGua -5' |
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| 11960 | 3' | -57.3 | NC_003278.1 | + | 13258 | 0.67 | 0.403012 |
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Target: 5'- cCGGGGCGcugccguugcccuGGGcGGcCugCACCGUCAc -3' miRNA: 3'- -GUCCUGC-------------UCCaCCaGugGUGGCAGUa -5' |
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| 11960 | 3' | -57.3 | NC_003278.1 | + | 22898 | 0.67 | 0.394589 |
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Target: 5'- -uGGGCGAGGacuucgaugcGGUgGCCACCGUgAa -3' miRNA: 3'- guCCUGCUCCa---------CCAgUGGUGGCAgUa -5' |
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| 11960 | 3' | -57.3 | NC_003278.1 | + | 6503 | 1.06 | 0.000581 |
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Target: 5'- cCAGGACGAGGUGGUCACCACCGUCAUg -3' miRNA: 3'- -GUCCUGCUCCACCAGUGGUGGCAGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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