Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11962 | 5' | -58.4 | NC_003278.1 | + | 27151 | 0.66 | 0.377443 |
Target: 5'- -gCUGGgaCUACCAgcucagccccUCGGGcCUCGAGCGg -3' miRNA: 3'- aaGACCg-GGUGGU----------AGCUC-GAGCUCGU- -5' |
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11962 | 5' | -58.4 | NC_003278.1 | + | 4535 | 0.66 | 0.350839 |
Target: 5'- -cCUGGCCgGCUggUGAGUUCG-GCGc -3' miRNA: 3'- aaGACCGGgUGGuaGCUCGAGCuCGU- -5' |
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11962 | 5' | -58.4 | NC_003278.1 | + | 4437 | 0.67 | 0.342272 |
Target: 5'- ---cGGCCgCAaCAUCGAGCgcgacuggaUCGAGCAg -3' miRNA: 3'- aagaCCGG-GUgGUAGCUCG---------AGCUCGU- -5' |
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11962 | 5' | -58.4 | NC_003278.1 | + | 10180 | 0.67 | 0.342272 |
Target: 5'- -aCUGGUUCACCAgcagCcAGC-CGAGCAg -3' miRNA: 3'- aaGACCGGGUGGUa---GcUCGaGCUCGU- -5' |
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11962 | 5' | -58.4 | NC_003278.1 | + | 22875 | 0.7 | 0.213844 |
Target: 5'- ---aGGCCCGCCAgCGccGGCgCGAGCAc -3' miRNA: 3'- aagaCCGGGUGGUaGC--UCGaGCUCGU- -5' |
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11962 | 5' | -58.4 | NC_003278.1 | + | 7118 | 1.05 | 0.000384 |
Target: 5'- cUUCUGGCCCACCAUCGAGCUCGAGCAu -3' miRNA: 3'- -AAGACCGGGUGGUAGCUCGAGCUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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