Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11965 | 5' | -52.5 | NC_003278.1 | + | 8227 | 0.66 | 0.731009 |
Target: 5'- aGCcagguuGGUCAGCCAGGgcacaCGGG-CGAggGCGa -3' miRNA: 3'- aCG------UCAGUUGGUCCa----GCUCaGCU--UGC- -5' |
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11965 | 5' | -52.5 | NC_003278.1 | + | 32977 | 0.66 | 0.731009 |
Target: 5'- cGguGUCGGCCAGuGcgCGgcacgacaacGGUCGAugGa -3' miRNA: 3'- aCguCAGUUGGUC-Ca-GC----------UCAGCUugC- -5' |
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11965 | 5' | -52.5 | NC_003278.1 | + | 26697 | 0.67 | 0.719885 |
Target: 5'- gGUuGUCGAacuucuUCAGGUCGAGgcccaggCGGGCGg -3' miRNA: 3'- aCGuCAGUU------GGUCCAGCUCa------GCUUGC- -5' |
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11965 | 5' | -52.5 | NC_003278.1 | + | 22136 | 0.67 | 0.708665 |
Target: 5'- --gGGUCGGCCuggcccauGGUCGAGggcacCGGACGc -3' miRNA: 3'- acgUCAGUUGGu-------CCAGCUCa----GCUUGC- -5' |
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11965 | 5' | -52.5 | NC_003278.1 | + | 21660 | 0.67 | 0.685991 |
Target: 5'- aGCAG-CGGCU-GGUCGAacGUCGGugGc -3' miRNA: 3'- aCGUCaGUUGGuCCAGCU--CAGCUugC- -5' |
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11965 | 5' | -52.5 | NC_003278.1 | + | 2312 | 0.67 | 0.674563 |
Target: 5'- cGCuGUCuauggaggggcuGGCCAagucGGUgGGGUCGAGCGu -3' miRNA: 3'- aCGuCAG------------UUGGU----CCAgCUCAGCUUGC- -5' |
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11965 | 5' | -52.5 | NC_003278.1 | + | 20085 | 0.68 | 0.651586 |
Target: 5'- cGCGG-CuGCCAGGUUGAGcaugcCGGGCa -3' miRNA: 3'- aCGUCaGuUGGUCCAGCUCa----GCUUGc -5' |
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11965 | 5' | -52.5 | NC_003278.1 | + | 16721 | 0.68 | 0.640062 |
Target: 5'- cGCGGUgacgugCGACCAGGgcgUCGGugucGUCGAGCu -3' miRNA: 3'- aCGUCA------GUUGGUCC---AGCU----CAGCUUGc -5' |
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11965 | 5' | -52.5 | NC_003278.1 | + | 10278 | 0.68 | 0.640062 |
Target: 5'- uUGCAGgacgauACCgAGGUCGAccuugcugcgGUCGAGCa -3' miRNA: 3'- -ACGUCagu---UGG-UCCAGCU----------CAGCUUGc -5' |
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11965 | 5' | -52.5 | NC_003278.1 | + | 6785 | 0.68 | 0.628531 |
Target: 5'- gGCAuGUCGGCCuGGUCGG--UGAGCa -3' miRNA: 3'- aCGU-CAGUUGGuCCAGCUcaGCUUGc -5' |
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11965 | 5' | -52.5 | NC_003278.1 | + | 23472 | 0.68 | 0.61585 |
Target: 5'- gUGUcGUCGGCCAGGUCGcugaccaGGuccUCGGGCa -3' miRNA: 3'- -ACGuCAGUUGGUCCAGC-------UC---AGCUUGc -5' |
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11965 | 5' | -52.5 | NC_003278.1 | + | 12872 | 0.69 | 0.594 |
Target: 5'- aGCGGUUcggacuCCAGGgCGAGgCGGGCGg -3' miRNA: 3'- aCGUCAGuu----GGUCCaGCUCaGCUUGC- -5' |
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11965 | 5' | -52.5 | NC_003278.1 | + | 14031 | 0.69 | 0.57114 |
Target: 5'- cGCAcgucGUCGAgCAGGcgcUCGAGcUCGAGCa -3' miRNA: 3'- aCGU----CAGUUgGUCC---AGCUC-AGCUUGc -5' |
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11965 | 5' | -52.5 | NC_003278.1 | + | 9282 | 0.69 | 0.57114 |
Target: 5'- gUGUAG-C-GCCGGGUCGGuGUCGAugaaGCGg -3' miRNA: 3'- -ACGUCaGuUGGUCCAGCU-CAGCU----UGC- -5' |
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11965 | 5' | -52.5 | NC_003278.1 | + | 22931 | 0.69 | 0.57114 |
Target: 5'- gGCGuGUCAG-CAGGUUGGcGUCGGACu -3' miRNA: 3'- aCGU-CAGUUgGUCCAGCU-CAGCUUGc -5' |
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11965 | 5' | -52.5 | NC_003278.1 | + | 4968 | 0.7 | 0.526143 |
Target: 5'- aGCcgAGuUCGGCCAGGUCGGcgggccGUCGaAGCGa -3' miRNA: 3'- aCG--UC-AGUUGGUCCAGCU------CAGC-UUGC- -5' |
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11965 | 5' | -52.5 | NC_003278.1 | + | 22849 | 0.7 | 0.526143 |
Target: 5'- cUGCAGcUgGACCAGGccaaCGAGUCcGACGc -3' miRNA: 3'- -ACGUC-AgUUGGUCCa---GCUCAGcUUGC- -5' |
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11965 | 5' | -52.5 | NC_003278.1 | + | 8240 | 0.71 | 0.451023 |
Target: 5'- gGCGGUCGcCUGGGUCGAcaagGUCGAcuucgACGa -3' miRNA: 3'- aCGUCAGUuGGUCCAGCU----CAGCU-----UGC- -5' |
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11965 | 5' | -52.5 | NC_003278.1 | + | 31642 | 0.71 | 0.451023 |
Target: 5'- cGCAGgcuUCAACCGGGcgcguccgCGGGUCGGcaACGu -3' miRNA: 3'- aCGUC---AGUUGGUCCa-------GCUCAGCU--UGC- -5' |
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11965 | 5' | -52.5 | NC_003278.1 | + | 6652 | 0.8 | 0.121089 |
Target: 5'- aGCGGUCGggcauGCCAGGUCGuGUgCGAGCa -3' miRNA: 3'- aCGUCAGU-----UGGUCCAGCuCA-GCUUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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