Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11966 | 3' | -51.3 | NC_003278.1 | + | 13313 | 0.66 | 0.839653 |
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Target: 5'- aCGggGCGacggUCGgcgUCGUUgagguaggCGUCGACg -3' miRNA: 3'- aGCuuCGCa---AGCa--GGCGAa-------GUAGCUG- -5' |
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| 11966 | 3' | -51.3 | NC_003278.1 | + | 12144 | 0.67 | 0.747894 |
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Target: 5'- gCGAcGGCGgca-UCCGCaUCGUCGGCg -3' miRNA: 3'- aGCU-UCGCaagcAGGCGaAGUAGCUG- -5' |
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| 11966 | 3' | -51.3 | NC_003278.1 | + | 4487 | 0.68 | 0.703148 |
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Target: 5'- cUCGAuguuGCGgcCGUCCGUggUCGccccuUCGACg -3' miRNA: 3'- -AGCUu---CGCaaGCAGGCGa-AGU-----AGCUG- -5' |
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| 11966 | 3' | -51.3 | NC_003278.1 | + | 31188 | 0.68 | 0.703148 |
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Target: 5'- gCG-GGUGUUUGUCCGCUgggcCAgggCGGCc -3' miRNA: 3'- aGCuUCGCAAGCAGGCGAa---GUa--GCUG- -5' |
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| 11966 | 3' | -51.3 | NC_003278.1 | + | 31146 | 0.7 | 0.576141 |
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Target: 5'- cUCGGccCGUUCGUUgGCUUgGUCGAUc -3' miRNA: 3'- -AGCUucGCAAGCAGgCGAAgUAGCUG- -5' |
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| 11966 | 3' | -51.3 | NC_003278.1 | + | 28845 | 0.72 | 0.497543 |
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Target: 5'- gCGAcGCGcUCGgCCGCgUCAUCGAUg -3' miRNA: 3'- aGCUuCGCaAGCaGGCGaAGUAGCUG- -5' |
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| 11966 | 3' | -51.3 | NC_003278.1 | + | 9203 | 1.12 | 0.001067 |
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Target: 5'- uUCGAAGCGUUCGUCCGCUUCAUCGACa -3' miRNA: 3'- -AGCUUCGCAAGCAGGCGAAGUAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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