Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11968 | 3' | -58.1 | NC_003278.1 | + | 6006 | 0.67 | 0.34624 |
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Target: 5'- cAUCCUGUCGCagaAGcgcaUGGGC-GGCCuGCc -3' miRNA: 3'- -UGGGAUAGCGg--UC----ACCCGaCUGGuCG- -5' |
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| 11968 | 3' | -58.1 | NC_003278.1 | + | 22676 | 0.68 | 0.321462 |
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Target: 5'- uGCCCuUGUCGaCCAGUccGCUGucacuCCAGCc -3' miRNA: 3'- -UGGG-AUAGC-GGUCAccCGACu----GGUCG- -5' |
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| 11968 | 3' | -58.1 | NC_003278.1 | + | 4687 | 0.68 | 0.329572 |
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Target: 5'- cGCCUgGUUGggcuCCAGuUGGGCgaagagGGCCAGCu -3' miRNA: 3'- -UGGGaUAGC----GGUC-ACCCGa-----CUGGUCG- -5' |
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| 11968 | 3' | -58.1 | NC_003278.1 | + | 2315 | 0.66 | 0.390498 |
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Target: 5'- cGCCgCUGUCuaUGGagGGGCUGGCCaAGUc -3' miRNA: 3'- -UGG-GAUAGcgGUCa-CCCGACUGG-UCG- -5' |
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| 11968 | 3' | -58.1 | NC_003278.1 | + | 19525 | 0.66 | 0.398847 |
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Target: 5'- gGCCCgguuguaGUCGCgGGUgagcgcgcgggugGGGUUG-CCGGCg -3' miRNA: 3'- -UGGGa------UAGCGgUCA-------------CCCGACuGGUCG- -5' |
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| 11968 | 3' | -58.1 | NC_003278.1 | + | 21739 | 0.66 | 0.428463 |
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Target: 5'- gGCCUg--CGCCGG-GGcGCUGgcGCCgAGCa -3' miRNA: 3'- -UGGGauaGCGGUCaCC-CGAC--UGG-UCG- -5' |
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| 11968 | 3' | -58.1 | NC_003278.1 | + | 19446 | 0.66 | 0.43829 |
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Target: 5'- -gCCUGUUgcgcgGCCAGcgcGGuGCUGACCuGCu -3' miRNA: 3'- ugGGAUAG-----CGGUCa--CC-CGACUGGuCG- -5' |
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| 11968 | 3' | -58.1 | NC_003278.1 | + | 30872 | 0.69 | 0.283165 |
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Target: 5'- gGCCgCUGUCGCgc-UGGGC-GAUCGGCa -3' miRNA: 3'- -UGG-GAUAGCGgucACCCGaCUGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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