Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11968 | 3' | -58.1 | NC_003278.1 | + | 21043 | 0.68 | 0.313503 |
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Target: 5'- gUCCUGgcaauugcCGCCGucGUGGcgguGCUGGCCGGCg -3' miRNA: 3'- uGGGAUa-------GCGGU--CACC----CGACUGGUCG- -5' |
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| 11968 | 3' | -58.1 | NC_003278.1 | + | 29872 | 0.68 | 0.305694 |
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Target: 5'- aGCCgCUguucaacgGUCGCgCGuUGGGCgUGACCGGCc -3' miRNA: 3'- -UGG-GA--------UAGCG-GUcACCCG-ACUGGUCG- -5' |
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| 11968 | 3' | -58.1 | NC_003278.1 | + | 23238 | 0.68 | 0.298035 |
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Target: 5'- uACgCgcUCGCCAG-GGGCcGGCCGGa -3' miRNA: 3'- -UGgGauAGCGGUCaCCCGaCUGGUCg -5' |
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| 11968 | 3' | -58.1 | NC_003278.1 | + | 30872 | 0.69 | 0.283165 |
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Target: 5'- gGCCgCUGUCGCgc-UGGGC-GAUCGGCa -3' miRNA: 3'- -UGG-GAUAGCGgucACCCGaCUGGUCG- -5' |
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| 11968 | 3' | -58.1 | NC_003278.1 | + | 33251 | 0.69 | 0.268888 |
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Target: 5'- cACCCUGUUGUUGGcUGaGGCUGGCgAGa -3' miRNA: 3'- -UGGGAUAGCGGUC-AC-CCGACUGgUCg -5' |
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| 11968 | 3' | -58.1 | NC_003278.1 | + | 22356 | 0.69 | 0.26197 |
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Target: 5'- cGCCC--UCGCCcgcGUgacGGGCuaccUGACCAGCg -3' miRNA: 3'- -UGGGauAGCGGu--CA---CCCG----ACUGGUCG- -5' |
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| 11968 | 3' | -58.1 | NC_003278.1 | + | 17612 | 0.7 | 0.228924 |
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Target: 5'- gACCCUgucgaacGUCGCCGucaacGGCgUGACCGGCa -3' miRNA: 3'- -UGGGA-------UAGCGGUcac--CCG-ACUGGUCG- -5' |
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| 11968 | 3' | -58.1 | NC_003278.1 | + | 9674 | 1.1 | 0.000226 |
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Target: 5'- aACCCUAUCGCCAGUGGGCUGACCAGCc -3' miRNA: 3'- -UGGGAUAGCGGUCACCCGACUGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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