Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11969 | 3' | -60.4 | NC_003278.1 | + | 6267 | 0.69 | 0.218007 |
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Target: 5'- --aGUCCCGCCaaGGCGCacuuCCUGC-GCg -3' miRNA: 3'- cgaCAGGGCGGcaCUGCG----GGACGuCG- -5' |
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| 11969 | 3' | -60.4 | NC_003278.1 | + | 8741 | 0.68 | 0.268097 |
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Target: 5'- cGCUGgCCCGaagaCCcUGGcCGCCCUcgGCGGCg -3' miRNA: 3'- -CGACaGGGC----GGcACU-GCGGGA--CGUCG- -5' |
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| 11969 | 3' | -60.4 | NC_003278.1 | + | 31148 | 0.67 | 0.303892 |
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Target: 5'- ----aCCCGCC-UGGCGCCCagcgcccucUGguGCg -3' miRNA: 3'- cgacaGGGCGGcACUGCGGG---------ACguCG- -5' |
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| 11969 | 3' | -60.4 | NC_003278.1 | + | 15346 | 0.66 | 0.319206 |
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Target: 5'- gGCUGguuaCCGgUGgcgGugGCCUgacgGCAGCa -3' miRNA: 3'- -CGACag--GGCgGCa--CugCGGGa---CGUCG- -5' |
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| 11969 | 3' | -60.4 | NC_003278.1 | + | 5889 | 0.66 | 0.327077 |
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Target: 5'- cCUGgcaCCGCCGUGACccggGCCUgGUGGUg -3' miRNA: 3'- cGACag-GGCGGCACUG----CGGGaCGUCG- -5' |
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| 11969 | 3' | -60.4 | NC_003278.1 | + | 31035 | 0.66 | 0.327077 |
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Target: 5'- cGCUGggCCGCaGcGACG-CUUGCAGCa -3' miRNA: 3'- -CGACagGGCGgCaCUGCgGGACGUCG- -5' |
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| 11969 | 3' | -60.4 | NC_003278.1 | + | 19347 | 0.66 | 0.327077 |
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Target: 5'- cGCgccUUCCGCacccagCGcGGCGCCCUGgAGCa -3' miRNA: 3'- -CGac-AGGGCG------GCaCUGCGGGACgUCG- -5' |
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| 11969 | 3' | -60.4 | NC_003278.1 | + | 19522 | 0.66 | 0.343249 |
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Target: 5'- ----aCCUGCagcacaGccACGCCCUGCAGCa -3' miRNA: 3'- cgacaGGGCGg-----CacUGCGGGACGUCG- -5' |
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| 11969 | 3' | -60.4 | NC_003278.1 | + | 22829 | 0.66 | 0.343249 |
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Target: 5'- cGCUGgcgggCCUGCCGa----UCCUGCAGCu -3' miRNA: 3'- -CGACa----GGGCGGCacugcGGGACGUCG- -5' |
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| 11969 | 3' | -60.4 | NC_003278.1 | + | 9770 | 1.11 | 0.000116 |
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Target: 5'- gGCUGUCCCGCCGUGACGCCCUGCAGCc -3' miRNA: 3'- -CGACAGGGCGGCACUGCGGGACGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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