Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 11970 | 3' | -56.2 | NC_003278.1 | + | 21437 | 0.71 | 0.300983 |
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Target: 5'- uGGGCGGguUCACCau---GGCCGGCc -3' miRNA: 3'- -CCUGCCguAGUGGaagcuCCGGCUGu -5' |
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| 11970 | 3' | -56.2 | NC_003278.1 | + | 2009 | 0.72 | 0.244651 |
|
Target: 5'- aGGGCaGCGUgAUCcaacugcgCGAGGCCGACAu -3' miRNA: 3'- -CCUGcCGUAgUGGaa------GCUCCGGCUGU- -5' |
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| 11970 | 3' | -56.2 | NC_003278.1 | + | 21759 | 0.73 | 0.225898 |
|
Target: 5'- cGGCGGCGacacuuacuacaUCACCaUCcAGGCCGGCGc -3' miRNA: 3'- cCUGCCGU------------AGUGGaAGcUCCGGCUGU- -5' |
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| 11970 | 3' | -56.2 | NC_003278.1 | + | 21625 | 0.74 | 0.180289 |
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Target: 5'- aGGGCGGgA--GCCUcgggaaacuccccgUCGAGGCCGGCGa -3' miRNA: 3'- -CCUGCCgUagUGGA--------------AGCUCCGGCUGU- -5' |
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| 11970 | 3' | -56.2 | NC_003278.1 | + | 543 | 0.75 | 0.16731 |
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Target: 5'- aGGACGGCGguagCACCcugUUGGGGCaguaCGACAu -3' miRNA: 3'- -CCUGCCGUa---GUGGa--AGCUCCG----GCUGU- -5' |
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| 11970 | 3' | -56.2 | NC_003278.1 | + | 10176 | 1.11 | 0.000387 |
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Target: 5'- aGGACGGCAUCACCUUCGAGGCCGACAu -3' miRNA: 3'- -CCUGCCGUAGUGGAAGCUCCGGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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